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PMID: 11101614 Published · ppublish English Journal Article

Characterization of non-type B Haemophilus influenzae strains isolated from patients with invasive disease. The HI Study Group.

Journal of clinical microbiology ·Vol. 38 ·No. 12 ·2000-12-00 ·Pages 4649-52

Cerquetti M, Ciofi degli Atti ML, Renna G, Tozzi AE, Garlaschi ML, Mastrantonio P

Abstract

Forty-one non-type b Haemophilus influenzae isolates from cases of invasive disease were characterized. By PCR capsular genotyping, 33 nonencapsulated strains, 4 type f isolates, and 4 b(-) strains were identified. By pulsed-field gel electrophoresis, the nonencapsulated isolates exhibited great genetic heterogenicity, whereas the type f and the b(-) strains seemed to have a clonal spread. Occurrence of the hifA gene was found by PCR in 18% of the nonencapsulated, 50% of the b(-), and all of the type f strains. Hemagglutinating fimbriae were generally expressed by nonencapsulated isolates when fimbrial gene hifA was present. Two nonencapsulated isolates not susceptible to ampicillin were detected; no strains were positive for beta-lactamase production.

MeSH Terms
Ampicillin/pharmacology Bacterial Capsules/genetics Bacterial Outer Membrane Proteins/genetics Electrophoresis, Gel, Pulsed-Field Fimbriae Proteins Fimbriae, Bacterial Genotype Haemophilus Infections/microbiology Haemophilus influenzae/classification,drug effects,isolation & purification Humans Microbial Sensitivity Tests Polymerase Chain Reaction beta-Lactamases/metabolism
Chemicals
Bacterial Outer Membrane Proteins HifA protein, Haemophilus influenzae Fimbriae Proteins Ampicillin beta-Lactamases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cerquetti M
Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy. [email protected]
Ciofi degli Atti M L
Renna G
Tozzi A E
Garlaschi M L
Mastrantonio P
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.38.12.4649-4652.2000
Email
[email protected]
Published
2000-12-00
Pages
4649-52
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC87655
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.38.12.4649-4652.2000 PMC Full Text View journal details
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