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PMID: 7867747 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction.

Epidemiology and infection ·Vol. 114 ·No. 1 ·1995-02-00 ·Pages 93-103

Hobson RP, Williams A, Rawal K, Pennington TH, Forbes KJ

Abstract

A PCR-based method of detecting Haemophilus influenzae in cultures inoculated from throat swabs was evaluated using samples from groups of laboratory staff and medical students and then applied to samples originating from the closed human community of an Antarctic research station. Suitable PCR primers to an H. influenzae gene (ompP2) were used to amplify the gene from DNA preparations made from mixed growth on chocolate agar with added vancomycin. PCR product was reamplified and subjected to restriction endonuclease digestion to allow temporal and spatial mapping of strains over an 8-month period. Eleven different strains of H. influenzae were detected. One particular strain was detected in a third of the base members.

MeSH Terms
Adult Antarctic Regions/epidemiology Base Sequence Ecological Systems, Closed Haemophilus Infections/epidemiology,microbiology,transmission Haemophilus influenzae/isolation & purification Humans Incidence Male Molecular Sequence Data Pharynx/microbiology Polymerase Chain Reaction/methods Restriction Mapping
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hobson R P
Department of Medical Microbiology, University of Aberdeen, Foresterhill.
Williams A
Rawal K
Pennington T H
Forbes K J
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Article Info
Journal
Epidemiology and infection
Abbr.
Epidemiol Infect
ISSN
0950-2688
DOI
10.1017/s0950268800051943
Published
1995-02-00
Pages
93-103
Language
English
Region
England
NLM ID
8703737
PMCID
PMC2271340
Subset
IM
External Links
PubMed source DOI: 10.1017/s0950268800051943 PMC Full Text View journal details
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