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PMID: 11102515 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Self-association of coilin reveals a common theme in nuclear body localization.

Molecular biology of the cell ·Vol. 11 ·No. 12 ·2000-12-00 ·Pages 4159-71

Hebert MD, Matera AG

Abstract

We have found that coilin, the marker protein for Cajal bodies (coiled bodies, CBs), is a self-interacting protein, and we have mapped the domain responsible for this activity to the amino-terminus. Together with a nuclear localization signal, the self-interaction domain is necessary and sufficient for localization to CBs. Overexpression of various wild-type and mutant coilin constructs in HeLa cells results in disruption of both CBs and survival motor neurons (SMN) gems. Additionally, we have identified a cryptic nucleolar localization signal (NoLS), within the coilin protein, which may be exposed in specific coilin phospho-isoforms. The implications of these findings are discussed in light of the fact that other proteins known to localize within nuclear bodies (e. g., PML, SMN and Sam68) can also self-associate. Thus protein self-interaction appears to be a general feature of nuclear body marker proteins.

MeSH Terms
Cell Nucleolus/metabolism Coiled Bodies/metabolism DNA Mutational Analysis HeLa Cells Humans Mutation Nuclear Localization Signals Nuclear Proteins/genetics,metabolism Phosphorylation Phosphoserine/metabolism Transfection
Chemicals
Nuclear Localization Signals Nuclear Proteins p80-coilin Phosphoserine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hebert M D
Department of Genetics, Center for Human Genetics and Program in Cell Biology, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio 44106-4955, USA.
Matera A G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.12.4159
Published
2000-12-00
Pages
4159-71
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC15064
Subset
IM
Grants
NIGMS NIH HHS · R01 GM053034 · United States
NICHD NIH HHS · T32 HD007518 · United States
NIGMS NIH HHS · GM-53034 · United States
NICHD NIH HHS · T32-HD07518 · United States
External Links
PubMed source DOI: 10.1091/mbc.11.12.4159 PMC Full Text View journal details
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