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PMID: 9679133 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nopp140 functions as a molecular link between the nucleolus and the coiled bodies.

The Journal of cell biology ·Vol. 142 ·No. 2 ·1998-07-27 ·Pages 319-29

Isaac C, Yang Y, Meier UT

Abstract

Coiled bodies are small nuclear organelles that are highly enriched in small nuclear RNAs, and that have long been thought to be associated with the nucleolus. Here we use mutational analysis, transient transfections, and the yeast two-hybrid system to show that the nucleolar phosphoprotein Nopp140 functions as a molecular link between the two prominent nuclear organelles. Exogenous Nopp140 accumulated in the nucleolus rapidly, but only after a lag phase in coiled bodies, suggesting a pathway between the two organelles. The expression of partial Nopp140 constructs exerted dominant negative effects on the endogenous Nopp140 by chasing it and other antigens that were common to both organelles out of the nucleolus. The alternating positively and negatively charged repeat domain of Nopp140 was required for targeting to both organelles. In addition, partial Nopp140 constructs caused formation of novel structures in the nucleoplasm and, in the case of the conserved carboxy terminus, led to the dispersal of coiled bodies. As a final link, we identified the coiled body-specific protein p80 coilin in a yeast two-hybrid screen with Nopp140. The interaction of the two proteins was confirmed by coimmunoprecipitation. Taken together, Nopp140 appeared to shuttle between the nucleolus and the coiled bodies, and to chaperone the transport of other molecules.

MeSH Terms
3T3 Cells Animals Binding Sites Biological Transport, Active COS Cells Cell Nucleolus/metabolism Cell Nucleus/metabolism,ultrastructure Fluorescent Antibody Technique, Indirect HeLa Cells Humans Mice Nuclear Proteins/genetics,metabolism Phosphoproteins/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Transfection
Chemicals
NOLC1 protein, human Nolc1 protein, mouse Nuclear Proteins Phosphoproteins Recombinant Fusion Proteins p80-coilin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Isaac C
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Yang Y
Meier U T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.142.2.319
Published
1998-07-27
Pages
319-29
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133063
Subset
IM
Grants
NIGMS NIH HHS · GM50725 · United States
External Links
PubMed source DOI: 10.1083/jcb.142.2.319 PMC Full Text View journal details
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