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PMID: 7841523 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis.

Molecular biology of the cell ·Vol. 5 ·No. 9 ·1994-09-00 ·Pages 955-66

Jiménez-García LF, Segura-Valdez ML, Ochs RL, Rothblum LI, Hannan R, Spector DL

Abstract

We have investigated the distribution of U3 snRNA and rRNA in HeLa cells and normal rat kidney cells during interphase and mitosis. U3 snRNA, known to be involved in pre-rRNA processing, was detected in nucleoli and coiled bodies during interphase, whereas rRNA was distributed in the nucleoli and throughout the cytoplasm. By comparison, ribosomal protein S6 was detected in nucleoli, coiled bodies, and in the cytoplasm. During nucleologenesis, pre-rRNA was observed in newly forming nucleoli during late telophase but not in prenucleolar bodies (PNBs), whereas U3 snRNA was detected in forming nucleoli and PNBs. Similar findings to those reported here for the localization of U3 snRNA have been reported previously for the U3 small nuclear ribonucleoprotein fibrillarin. These results suggest that components involved in pre-rRNA processing localize to discrete PNBs at the end of mitosis. The nucleolus is formed at specific telophase domains (nucleolar organizing regions) and the PNBs, containing factors essential for pre-rRNA processing, are recruited to these sites of rRNA transcription and processing.

MeSH Terms
Animals Cell Nucleolus/metabolism,ultrastructure Cells, Cultured HeLa Cells Humans Interphase Kidney Mitosis RNA Polymerase I/metabolism RNA Precursors/metabolism RNA, Small Nuclear/metabolism Rats Transcription, Genetic
Chemicals
RNA Precursors RNA, Small Nuclear RNA Polymerase I
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jiménez-García L F
Laboratory of Electron Microscopy, Faculty of Sciences, National Autonomous University of Mexico, Mexico, D.F.
Segura-Valdez M L
Ochs R L
Rothblum L I
Hannan R
Spector D L
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.5.9.955
Published
1994-09-00
Pages
955-66
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301119
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042694 · United States
NCI NIH HHS · 5P30CA-45508-06 · United States
NIGMS NIH HHS · GM-42694 · United States
External Links
PubMed source DOI: 10.1091/mbc.5.9.955 PMC Full Text View journal details
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