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PMID: 1756730 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.

The EMBO journal ·Vol. 10 ·No. 13 ·1991-12-00 ·Pages 4231-9

Hughes JM, Ares M

Abstract

Multiple processing events are required to convert a single eukaryotic pre-ribosomal RNA (pre-rRNA) into mature 18S (small subunit), 5.8S and 25-28S (large subunit) rRNAs. We have asked whether U3 small nucleolar RNA is required for pre-rRNA processing in vivo by depleting Saccharomyces cerevisiae of U3 by conditional repression of U3 synthesis. The resulting pattern of accumulation and depletion of specific pre-rRNAs indicates that U3 is required for multiple events leading to the maturation of 18S rRNA. These include an initial cleavage within the 5' external transcribed spacer, resembling the U3 dependent initial processing event of mammalian pre-rRNA. Formation of large subunit rRNAs is unaffected by U3 depletion. The similarity between the effects of U3 depletion and depletion of U14 small nucleolar RNA and the nucleolar protein fibrillarin (NOP1) suggests that these could be components of a single highly conserved processing complex.

MeSH Terms
Animals Base Sequence Blotting, Northern Humans Molecular Sequence Data Plasmids RNA Precursors/genetics,metabolism RNA, Ribosomal, 18S/antagonists & inhibitors,biosynthesis,genetics RNA, Small Nuclear/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/metabolism Sequence Homology, Nucleic Acid Templates, Genetic Transcription, Genetic
Chemicals
RNA Precursors RNA, Ribosomal, 18S RNA, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hughes J M
Sinsheimer Laboratories, University of California, Santa Cruz 95064.
Ares M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-12-00
Pages
4231-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453175
Subset
IM
Grants
NIGMS NIH HHS · GM40478 · United States
External Links
PubMed source PMC Full Text View journal details
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