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PMID: 11121446 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex.

The Journal of cell biology ·Vol. 151 ·No. 6 ·2000-12-11 ·Pages 1337-44

Heil-Chapdelaine RA, Oberle JR, Cooper JA

Abstract

In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dynein-dependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud.

MeSH Terms
Actins/metabolism Calcium-Binding Proteins/metabolism Cell Compartmentation Chromosome Segregation Cytoskeletal Proteins Dyneins/metabolism Fungal Proteins/metabolism Kinesins Microscopy/methods Microtubule-Associated Proteins/metabolism Microtubules/metabolism Mitosis/genetics,physiology Movement Nuclear Proteins/metabolism Phenotype Saccharomyces cerevisiae Proteins Saccharomycetales Spindle Apparatus/metabolism
Chemicals
Actins Calcium-Binding Proteins Cytoskeletal Proteins Fungal Proteins KAR9 protein, S cerevisiae KIP3 protein, S cerevisiae Microtubule-Associated Proteins NUM1 protein, S cerevisiae Nuclear Proteins Saccharomyces cerevisiae Proteins Dyneins Kinesins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heil-Chapdelaine R A
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Oberle J R
Cooper J A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.151.6.1337
Published
2000-12-11
Pages
1337-44
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2190597
Subset
IM
Grants
NIGMS NIH HHS · R01 GM047337 · United States
NIGMS NIH HHS · R01 GM047337-09 · United States
NIGMS NIH HHS · GM47337 · United States
External Links
PubMed source DOI: 10.1083/jcb.151.6.1337 PMC Full Text View journal details
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