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PMID: 9201714 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways.

Molecular biology of the cell ·Vol. 8 ·No. 6 ·1997-06-00 ·Pages 1035-50

Geiser JR, Schott EJ, Kingsbury TJ, Cole NB, Totis LJ, Bhattacharyya G, He L, Hoyt MA

Abstract

Kinesin-related Cin8p is the most important spindle-pole-separating motor in Saccharomyces cerevisiae but is not essential for cell viability. We identified 20 genes whose products are specifically required by cell deficient for Cin8p. All are associated with mitotic roles and represent at least four different functional pathways. These include genes whose products act in two spindle motor pathways that overlap in function with Cin8p, the kinesin-related Kip1p pathway and the cytoplasmic dynein pathway. In addition, genes required for mitotic spindle checkpoint function and for normal microtubule stability were recovered. Mutant alleles of eight genes caused phenotypes similar to dyn1 (encodes the dynein heavy chain), including a spindle-positioning defect. We provide evidence that the products of these genes function in concept with dynein. Among the dynein pathway gene products, we found homologues of the cytoplasmic dynein intermediate chain, the p150Glued subunit of the dynactin complex, and human LIS-1, required for normal brain development. These findings illustrate the complex cellular interactions exhibited by Cin8p, a member of a conserved spindle motor family.

MeSH Terms
Amino Acid Sequence Cell Movement Cell Nucleus/ultrastructure Cloning, Molecular DNA-Binding Proteins/genetics Dyneins/physiology Endoribonucleases Fungal Proteins/genetics,physiology Gene Deletion Genes, Fungal Genetic Complementation Test Kinesins Microtubule-Associated Proteins/genetics,physiology Microtubules/physiology,ultrastructure Mitosis Molecular Motor Proteins Molecular Sequence Data Mutagenesis Phenotype Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spindle Apparatus/physiology Temperature
Chemicals
CIN8 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins JNM1 protein, S cerevisiae KIP1 protein, S cerevisiae Microtubule-Associated Proteins Molecular Motor Proteins Saccharomyces cerevisiae Proteins Endoribonucleases PAC1 protein, S cerevisiae Dyneins Kinesins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Geiser J R
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Schott E J
Kingsbury T J
Cole N B
Totis L J
Bhattacharyya G
He L
Hoyt M A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.8.6.1035
Published
1997-06-00
Pages
1035-50
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC305712
Subset
IM
Grants
NIGMS NIH HHS · GM-40714 · United States
External Links
PubMed source DOI: 10.1091/mbc.8.6.1035 PMC Full Text View journal details
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