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PMID: 8028580 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificity kinases.

Molecular & general genetics : MGG ·Vol. 243 ·No. 6 ·1994-06-15 ·Pages 641-53

Poch O, Schwob E, de Fraipont F, Camasses A, Bordonné R, Martin RP

Abstract

We report here the sequence of RPK1 (for Regulatory cell Proliferation Kinase), a new Saccharomyces cerevisiae gene coding for a protein with sequence similarities to serine/threonine protein kinases. The protein sequence of 764 amino acids includes an amino-terminal domain (residues 1-410), which may be involved in regulation of the kinase domain (residues 411-764). The catalytic domain of Rpk1 is not closely related to other known yeast protein kinases but exhibits strong homology to a newly discovered group of mammalian kinases (PYT, TTK, esk) with serine/threonine/tyrosine kinase activity. Null alleles of RPK1 are lethal and thus this gene belongs to the small group of yeast protein kinase genes that are essential for cell growth. In addition, eliminating the expression of RPK1 gives rise to the accumulation of non-viable cells with less than a 1 N DNA content suggesting that cells proceed into mitosis without completion of DNA synthesis. Therefore, the Rpk1 kinase may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated a cells and increased late in meiosis in a/alpha diploids. Taken together, our results suggest that Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Differentiation/genetics Cell Division/genetics DNA, Fungal/biosynthesis Gene Expression Regulation, Fungal Genes, Fungal/genetics Genes, Lethal/genetics Mating Factor Meiosis/genetics Mitosis/genetics Molecular Sequence Data Peptides/genetics Pheromones/pharmacology Protein Serine-Threonine Kinases/genetics Protein-Tyrosine Kinases/genetics RNA, Messenger/biosynthesis Saccharomyces cerevisiae/cytology,drug effects,genetics Saccharomyces cerevisiae Proteins Sequence Analysis Sequence Homology, Amino Acid Substrate Specificity
Chemicals
DNA, Fungal Peptides Pheromones RNA, Messenger Saccharomyces cerevisiae Proteins Mating Factor Protein-Tyrosine Kinases Protein Serine-Threonine Kinases MPS1 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Poch O
Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Strasbourg, France.
Schwob E
de Fraipont F
Camasses A
Bordonné R
Martin R P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00279573
Published
1994-06-15
Pages
641-53
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S71711
External Links
PubMed source DOI: 10.1007/BF00279573
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