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PMID: 11238921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7.

Molecular and cellular biology ·Vol. 21 ·No. 5 ·2001-03-00 ·Pages 1841-53

Gangloff YG, Sanders SL, Romier C, Kirschner D, Weil PA, Tora L, Davidson I

Abstract

We show that the yeast TFIID (yTFIID) component yTAF(II)47 contains a histone fold domain (HFD) with homology to that previously described for hTAF(II)135. Complementation in vivo indicates that the yTAF(II)47 HFD is necessary and sufficient for vegetative growth. Mutation of highly conserved residues in the alpha1 helix of the yTAF(II)47 HFD results in a temperature-sensitive phenotype which can be suppressed by overexpression of yTAF(II)25, as well as by yTAF(II)40, yTAF(II)19, and yTAF(II)60. In yeast two-hybrid and bacterial coexpression assays, the yTAF(II)47 HFD selectively heterodimerizes with yTAF(II)25, which we show contains an HFD with homology to the hTAF(II)28 family We additionally demonstrate that yTAF(II)65 contains a functional HFD which also selectively heterodimerizes with yTAF(II)25. These results reveal the existence of two novel histone-like pairs in yTFIID. The physical and genetic interactions described here show that the histone-like yTAF(II)s are organized in at least two substructures within TFIID rather than in a single octamer-like structure as previously suggested. Furthermore, our results indicate that ySPT7 has an HFD homologous to that of yTAF(II)47 which selectively heterodimerizes with yTAF(II)25, defining a novel histone-like pair in the SAGA complex.

MeSH Terms
Amino Acid Sequence Cell Division Dimerization Genetic Complementation Test Histones/chemistry Lac Operon Models, Genetic Molecular Sequence Data Phenotype Plasmids/metabolism Protein Folding Protein Structure, Tertiary Recombinant Proteins/metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Temperature Transcription Factor TFIID Transcription Factors/metabolism Transcription Factors, TFII/chemistry,genetics,metabolism Two-Hybrid System Techniques beta-Galactosidase/metabolism
Chemicals
Histones Recombinant Proteins SPT7 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factor TFIID Transcription Factors Transcription Factors, TFII beta-Galactosidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gangloff Y G
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch Cédex, C.U. de Strasbourg, France.
Sanders S L
Romier C
Kirschner D
Weil P A
Tora L
Davidson I
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.5.1841-1853.2001
Published
2001-03-00
Pages
1841-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC86751
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052461 · United States
NIGMS NIH HHS · R56 GM052461 · United States
NIGMS NIH HHS · GM52461 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.5.1841-1853.2001 PMC Full Text View journal details
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