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PMID: 11239004 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The N- and C-terminal regions of RBP-J interact with the ankyrin repeats of Notch1 RAMIC to activate transcription.

Nucleic acids research ·Vol. 29 ·No. 6 ·2001-03-15 ·Pages 1373-80

Tani S, Kurooka H, Aoki T, Hashimoto N, Honjo T

Abstract

The evolutionarily-conserved DNA-binding protein RBP-J directly interacts with the RAM domain and the ankyrin (ANK) repeats of the Notch intracellular region (RAMIC), and activates transcription of downstream target genes that regulate cell differentiation. In vitro binding assays demonstrate that the truncated N- and C-terminal regions of RBP-J bind to the ANK repeats but not to the RAM domain. Using an OT11 mouse cell line, in which the RBP-J locus is disrupted, we showed that RBP-J constructs mutated in the N- and C-terminal regions were defective in their transcriptional activation induced by either RAMIC or IC (the Notch intracellular region without the RAM domain) although they had normal levels of binding activity to DNA and the RAM domain. The studies using chimeric molecules between RBP-J and its homolog RBP-L showed that the N- and C-terminal regions of RBP-J conferred the IC- as well as RAMIC-induced transactivation potential on RBP-L, which binds to the same DNA sequence as RBP-J but fails to interact with RAMIC. Taken together, these results indicate that the interactions between the N- and C-terminal regions of RBP-J and the ANK repeats of RAMIC are important for transactivation of RBP-J by RAMIC.

MeSH Terms
Animals Ankyrin Repeat Binding Sites Binding, Competitive Cell Line DNA-Binding Proteins/chemistry,genetics,metabolism Membrane Proteins/genetics,metabolism Mutation Nuclear Proteins Protein Binding Receptor, Notch1 Receptors, Cell Surface Recombinant Fusion Proteins/genetics,metabolism Transcription Factors Transcriptional Activation
Chemicals
DNA-Binding Proteins Membrane Proteins Nuclear Proteins Rbpsuhl protein, mouse Receptor, Notch1 Receptors, Cell Surface Recombinant Fusion Proteins Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tani S
Department of Medical Chemistry and Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
Kurooka H
Aoki T
Hashimoto N
Honjo T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/29.6.1373
Published
2001-03-15
Pages
1373-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC29757
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/29.6.1373 PMC Full Text View journal details
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