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PMID: 11274109 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis.

Journal of bacteriology ·Vol. 183 ·No. 8 ·2001-04-00 ·Pages 2497-504

Ali NO, Bignon J, Rapoport G, Debarbouille M

Abstract

Bacillus subtilis grown in media containing amino acids or glucose secretes acetate, pyruvate, and large quantities of acetoin into the growth medium. Acetoin can be reused by the bacteria during stationary phase when other carbon sources have been depleted. The acoABCL operon encodes the E1alpha, E1beta, E2, and E3 subunits of the acetoin dehydrogenase complex in B. subtilis. Expression of this operon is induced by acetoin and repressed by glucose in the growth medium. The acoR gene is located downstream from the acoABCL operon and encodes a positive regulator which stimulates the transcription of the operon. The product of acoR has similarities to transcriptional activators of sigma 54-dependent promoters. The four genes of the operon are transcribed from a -12, -24 promoter, and transcription is abolished in acoR and sigL mutants. Deletion analysis showed that DNA sequences more than 85 bp upstream from the transcriptional start site are necessary for full induction of the operon. These upstream activating sequences are probably the targets of AcoR. Analysis of an acoR'-'lacZ strain of B. subtilis showed that the expression of acoR is not induced by acetoin and is repressed by the presence of glucose in the growth medium. Transcription of acoR is also negatively controlled by CcpA, a global regulator of carbon catabolite repression. A specific interaction of CcpA in the upstream region of acoR was demonstrated by DNase I footprinting experiments, suggesting that repression of transcription of acoR is mediated by the binding of CcpA to the promoter region of acoR.

MeSH Terms
Acetoin/metabolism Acetoin Dehydrogenase/genetics,metabolism Bacillus subtilis/genetics,growth & development,metabolism Bacterial Proteins Base Sequence Culture Media DNA Footprinting DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Glucose/metabolism Molecular Sequence Data Operon Plasmids Promoter Regions, Genetic Repressor Proteins/genetics,metabolism Sigma Factor/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
Bacterial Proteins Culture Media DNA-Binding Proteins Repressor Proteins Sigma Factor Transcription Factors catabolite control proteins, bacteria ACOR protein, Alcaligenes eutrophus Acetoin Acetoin Dehydrogenase Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ali N O
Unité de Biochimie Microbienne, Institut Pasteur, URA 2172 du Centre National de la Recherche Scientifique, 75724 Paris Cedex 15, France.
Bignon J
Rapoport G
Debarbouille M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.8.2497-2504.2001
Published
2001-04-00
Pages
2497-504
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95166
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.183.8.2497-2504.2001 PMC Full Text View journal details
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