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PMID: 11274117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic isolation of meningococci of the electrophoretic type 37 complex.

Journal of bacteriology ·Vol. 183 ·No. 8 ·2001-04-00 ·Pages 2570-5

Claus H, Stoevesandt J, Frosch M, Vogel U

Abstract

Neisseria meningitidis (the meningococcus) is a naturally competent bacterial species in which intra- and interspecific horizontal gene transfer is a major source of genetic diversity. In strains of the electrophoretic type 37 (ET-37) complex and of the A4 cluster, we identified genomic DNA coding for a novel restriction-modification system and for the tail of a previously unidentified prophage. Furthermore, a novel 7.2-kb DNA segment restricted to clones of the ET-37 complex and the A4 cluster was isolated and shown to occur both as a plasmid (pJS-B) and as a chromosomal integration. Neither the genomic loci nor pJS-B was present in ET-5 complex, lineage 3, or serogroup A meningococci. The differential distribution of the DNA segments described herein, as well as of opcA, porB, nmeAI, nmeBI, and nmeDI described previously, supports the concept of genetic isolation of hypervirulent lineages responsible for most cases of serogroup C disease worldwide.

MeSH Terms
Bacteriophages/genetics Chromosome Mapping DNA Restriction-Modification Enzymes/metabolism Electrophoresis/methods Enzymes/analysis Genome, Bacterial Humans Molecular Sequence Data Neisseria meningitidis/classification,genetics,virology Nucleic Acid Hybridization Plasmids/genetics Sequence Analysis, DNA
Chemicals
DNA Restriction-Modification Enzymes Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Claus H
Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany.
Stoevesandt J
Frosch M
Vogel U
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.8.2570-2575.2001
Published
2001-04-00
Pages
2570-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95174
Subset
IM
Databases
GENBANK
AJ277475, AJ278707, AJ278708
External Links
PubMed source DOI: 10.1128/JB.183.8.2570-2575.2001 PMC Full Text View journal details
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