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PMID: 11416180 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Assembly and transport of a premessenger RNP particle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 13 ·2001-06-19 ·Pages 7012-7

Daneholt B

Abstract

Salivary gland cells in the larvae of the dipteran Chironomus tentans offer unique possibilities to visualize the assembly and nucleocytoplasmic transport of a specific transcription product. Each nucleus harbors four giant polytene chromosomes, whose transcription sites are expanded, or puffed. On chromosome IV, there are two puffs of exceptional size, Balbiani ring (BR) 1 and BR 2. A BR gene is 35-40 kb, contains four short introns, and encodes a 1-MDa salivary polypeptide. The BR transcript is packed with proteins into a ribonucleoprotein (RNP) fibril that is folded into a compact ring-like structure. The completed RNP particle is released into the nucleoplasm and transported to the nuclear pore, where the RNP fibril is gradually unfolded and passes through the pore. On the cytoplasmic side, the exiting extended RNP fibril becomes engaged in protein synthesis and the ensuing polysome is anchored to the endoplasmic reticulum. Several of the BR particle proteins have been characterized, and their fate during the assembly and transport of the BR particle has been elucidated. The proteins studied are all added cotranscriptionally to the pre-mRNA molecule. The various proteins behave differently during RNA transport, and the flow pattern of each protein is related to the particular function of the protein. Because the cotranscriptional assembly of the pre-mRNP particle involves proteins functioning in the nucleus as well as proteins functioning in the cytoplasm, it is concluded that the fate of the mRNA molecule is determined to a considerable extent already at the gene level.

MeSH Terms
Animals Cell Nucleus/metabolism Chironomidae/genetics,physiology Chromosome Mapping Larva Protein Transport RNA-Binding Proteins/metabolism Ribonucleoproteins/genetics,metabolism Salivary Proteins and Peptides/genetics,physiology Spliceosomes/physiology,ultrastructure
Chemicals
RNA-Binding Proteins Ribonucleoproteins Salivary Proteins and Peptides
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Daneholt B
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, Box 285, SE-17177 Stockholm, Sweden. [email protected]
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.111145498
Email
[email protected]
Published
2001-06-19
Pages
7012-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC34615
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.111145498 PMC Full Text View journal details
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