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PMID: 11553789 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of yeast DNA topoisomerase III and Sgs1 DNA helicase: studies of fusion proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 20 ·2001-09-25 ·Pages 11108-13

Bennett RJ, Wang JC

Abstract

The Sgs1 protein of the budding yeast Saccharomyces cerevisiae is a member of the RecQ DNA helicase family that includes the human Bloom, Werner, and Rothmund-Thompson syndrome proteins. The N-terminal region outside the central DNA helicase core of Sgs1, particularly the part containing the first 100 amino acid residues of the 1,447-residue protein, is known to be functionally important and has been implicated in Sgs1-DNA topoisomerase III (Top3) interaction. We show in this work that the functionality of a truncated Sgs1 lacking its N-terminal 106 residues can be restored by replacing the truncated region with Top3. Fusion of Top3 to a mutant Sgs1 with a Val-29 to Glu substitution, which interferes with Sgs1-Top3 interaction, similarly restores the functionality of the mutant Sgs1(V29E) protein. The Top3-Sgs1(Delta1-106) and Top3-Sgs1(V29E) fusion proteins behave like wild-type Sgs1 in complementing several aspects of the sgs1 phenotype, including the hypersensitivity of sgs1 cells to methyl methanesulfonate and hydroxyurea. Complementation by the fusion proteins required both the topoisomerase activity of Top3 and the helicase activity of the Sgs1 polypeptide. These results suggest that the sole function of the N-terminal 106 amino acid residues of Sgs1 is for Top3 binding, and that the coordinated actions of Sgs1 and Top3 are important in cellular processes such as the processing of DNA after exposure of cells to DNA-damaging agents.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Amino Acid Substitution Binding Sites Cloning, Molecular DNA Helicases/chemistry,genetics,metabolism DNA Topoisomerases, Type I/chemistry,genetics,metabolism Gene Deletion Genetic Complementation Test Humans Hydroxyurea/pharmacology Methyl Methanesulfonate/pharmacology Mutagenesis Mutagenesis, Site-Directed RecQ Helicases Recombinant Fusion Proteins/chemistry,metabolism Saccharomyces cerevisiae/drug effects,enzymology,genetics Saccharomyces cerevisiae Proteins Sequence Deletion
Chemicals
Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Methyl Methanesulfonate Adenosine Triphosphatases RECQL protein, human SGS1 protein, S cerevisiae DNA Helicases RecQ Helicases DNA Topoisomerases, Type I Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bennett R J
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Wang J C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.201387098
Published
2001-09-25
Epub
2001-00-11
Pages
11108-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC58691
Subset
IM
Grants
NIGMS NIH HHS · R01 GM024544 · United States
NIGMS NIH HHS · R37 GM024544 · United States
NIGMS NIH HHS · GM24544 · United States
External Links
PubMed source DOI: 10.1073/pnas.201387098 PMC Full Text View journal details
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