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PMID: 11579156 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rapidly inactivating and non-inactivating calcium-activated potassium currents in frog saccular hair cells.

The Journal of physiology ·Vol. 536 ·No. Pt 1 ·2001-10-01 ·Pages 49-65

Armstrong CE, Roberts WM

Abstract

1. Using a semi-intact epithelial preparation we examined the Ca(2+)-activated K(+) (K(Ca)) currents of frog (Rana pipiens) saccular hair cells. After blocking voltage-dependent K(+) (K(V)) currents with 4-aminopyridine (4-AP) an outward current containing inactivating (I(transient)) and non-inactivating (I(steady)) components remained. 2. The contribution of each varied greatly from cell to cell, with I(transient) contributing from 14 to 90 % of the total outward current. Inactivation of I(transient) was rapid (tau approximately 2-3 ms) and occurred within the physiological range of membrane potentials (V(1/2) = -63 mV). Recovery from inactivation was also rapid (tau approximately 10 ms). 3. Suppression of both I(transient) and I(steady) by depolarizations that approached the Ca(2+) equilibrium potential and by treatments that blocked Ca(2+) influx (application Ca(2+)-free saline or Cd(2+)), suggest both are Ca(2+) dependent. Both were blocked by iberiotoxin, a specific blocker of large-conductance K(Ca) channels (BK), but not by apamin, a specific blocker of small-conductance K(Ca) channels. 4. Ensemble-variance analysis showed that I(transient) and I(steady) flow through two distinct populations of channels, both of which have a large single-channel conductance (~100 pS in non-symmetrical conditions). Together, these data indicate that both I(transient) and I(steady) are carried through BK channels, one of which undergoes rapid inactivation while the other does not. 5. Inactivation of I(transient) could be removed by extracellular papain and could later be restored by intracellular application of the 'ball' domain of the auxiliary subunit (beta2) thought to mediate BK channel inactivation in rat chromaffin cells. We hypothesize that I(transient) results from the association of a similar beta subunit with some of the BK channels and that papain removes inactivation by cleaving extracellular sites required for this association.

MeSH Terms
4-Aminopyridine/pharmacology Animals Apamin/pharmacology Artifacts Hair Cells, Auditory/physiology Ion Channel Gating/drug effects,physiology Large-Conductance Calcium-Activated Potassium Channel beta Subunits Large-Conductance Calcium-Activated Potassium Channels Papain/pharmacology Patch-Clamp Techniques Peptides/pharmacology Potassium/metabolism Potassium Channel Blockers/pharmacology Potassium Channels/chemistry,metabolism Potassium Channels, Calcium-Activated/chemistry,metabolism Protein Structure, Tertiary Rana pipiens Saccule and Utricle/cytology,physiology Tetraethylammonium/pharmacology
Chemicals
Large-Conductance Calcium-Activated Potassium Channel beta Subunits Large-Conductance Calcium-Activated Potassium Channels Peptides Potassium Channel Blockers Potassium Channels Potassium Channels, Calcium-Activated Apamin Tetraethylammonium iberiotoxin 4-Aminopyridine Papain Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Armstrong C E
Institute of Neuroscience, University of Oregon, Eugene, 97403-1254, USA.
Roberts W M
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1111/j.1469-7793.2001.00049.x
Published
2001-10-01
Pages
49-65
Language
English
Region
England
NLM ID
0266262
PMCID
PMC2278855
Subset
IM
Grants
NINDS NIH HHS · R01 NS027142 · United States
NIGMS NIH HHS · T32 GM007257 · United States
NIGMS NIH HHS · GM07257 · United States
NINDS NIH HHS · NS27142 · United States
External Links
PubMed source DOI: 10.1111/j.1469-7793.2001.00049.x PMC Full Text View journal details
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