Home LiteratureArticle Details
PMID: 11901113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic and physical mapping of Avr1a in Phytophthora sojae.

Genetics ·Vol. 160 ·No. 3 ·2002-03-00 ·Pages 949-59

MacGregor T, Bhattacharyya M, Tyler B, Bhat R, Schmitthenner AF, Gijzen M

Abstract

The interaction between soybean and the phytopathogenic oomycete Phytophthora sojae is controlled by host resistance (Rps) genes and pathogen avirulence (Avr) genes. We have mapped the Avr1a locus in F(2) populations derived from four different P. sojae races. Four RAPD and nine AFLP markers linked to Avr1a were initially identified. Nine markers were used to compare genetic linkage maps of the Avr1a locus in two distinct F(2) populations. Distorted segregation ratios favoring homozygous genotypes were noted in both crosses. Segregation analysis of all the markers in one F(2) population of 90 progeny generated a map of 113.2 cM encompassing Avr1a, with one marker cosegregating with the gene. The cosegregating DNA marker was used to isolate P. sojae BAC clones and construct a physical map covering 170 kb, from which additional DNA markers were developed. Three markers occurring within the BAC contig were mapped in an enlarged population of 486 F(2) progeny. Avr1a was localized to a 114-kb interval, and an average physical to genetic distance ratio of 391 kb/cM was calculated for this region. This work provides a basis for the positional cloning of Avr1a.

MeSH Terms
Algal Proteins/genetics Chromosome Mapping Chromosomes, Artificial, Bacterial Crosses, Genetic Fungal Proteins/genetics Genes, Dominant Genes, Fungal Genetic Markers Phytophthora/genetics,pathogenicity Virulence
Chemicals
Algal Proteins Fungal Proteins Genetic Markers
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
MacGregor Terry
Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.
Bhattacharyya Madan
Tyler Brett
Bhat Ravindra
Schmitthenner August F
Gijzen Mark
References (19)
19 references, click to expand
  1. Mapping of avirulence genes in Phytophthora infestans with amplified fragment length polymorphism markers selected by bulked segregant analysis.
    Genetics. 2001 Mar;157(3):949-56 PMID: 11238385
  2. The phytophthora genome initiative database: informatics and analysis for distributed pathogenomic research.
    Nucleic Acids Res. 2000 Jan 1;28(1):87-90 PMID: 10592189
  3. Bacterial avirulence genes.
    Annu Rev Phytopathol. 1996;34:153-79 PMID: 15012539
  4. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828-32 PMID: 1682921
  5. AFLP: a new technique for DNA fingerprinting.
    Nucleic Acids Res. 1995 Nov 11;23(21):4407-14 PMID: 7501463
  6. Initial assessment of gene diversity for the oomycete pathogen Phytophthora infestans based on expressed sequences.
    Fungal Genet Biol. 1999 Nov;28(2):94-106 PMID: 10587472
  7. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
    Nucleic Acids Res. 1990 Nov 25;18(22):6531-5 PMID: 1979162
  8. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.
    Genomics. 1987 Oct;1(2):174-81 PMID: 3692487
  9. Evidence for outcrossing in Phytophthora sojae and linkage of a DNA marker to two avirulence genes.
    Curr Genet. 1994 Dec;27(1):77-82 PMID: 7750150
  10. Avirulence and resistance genes in the Cladosporium fulvum-tomato interaction.
    Curr Opin Microbiol. 1999 Aug;2(4):368-73 PMID: 10458978
  11. Fingerprinting genomes using PCR with arbitrary primers.
    Nucleic Acids Res. 1990 Dec 25;18(24):7213-8 PMID: 2259619
  12. AFLP Linkage Map of the Oomycete Phytophthora infestans
    Fungal Genet Biol. 1997 Jun;21(3):278-91 PMID: 9299198
  13. Resistance of nicotiana benthamiana to phytophthora infestans is mediated by the recognition of the elicitor protein INF1
    Plant Cell. 1998 Sep;10(9):1413-26 PMID: 9724689
  14. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.
    Plant J. 1993 Aug;4(2):403-10 PMID: 8106085
  15. Phytophthora sojae avirulence genes, RAPD, and RFLP markers used to construct a detailed genetic linkage map.
    Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):988-95 PMID: 8664507
  16. Pooled-sampling makes high-resolution mapping practical with DNA markers.
    Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):16-20 PMID: 8419918
  17. Integration of CAPS markers into the RFLP map generated using recombinant inbred lines of Arabidopsis thaliana.
    Plant Mol Biol. 1994 Feb;24(4):685-7 PMID: 7908836
  18. Comparative analysis of expressed sequences in Phytophthora sojae.
    Plant Physiol. 2000 May;123(1):243-54 PMID: 10806241
  19. Genome size determination in peronosporales (Oomycota) by Feulgen image analysis.
    Fungal Genet Biol. 1998 Dec;25(3):181-95 PMID: 9917372
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/160.3.949
Published
2002-03-00
Pages
949-59
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462023
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/160.3.949 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2