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PMID: 11927603 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115.

The Journal of cell biology ·Vol. 157 ·No. 1 ·2002-04-01 ·Pages 45-62

Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G

Abstract

p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).

MeSH Terms
Animals Autoantigens COP-Coated Vesicles/metabolism Carrier Proteins/metabolism Detergents Golgi Apparatus/metabolism Golgi Matrix Proteins In Vitro Techniques Membrane Fusion/physiology Membrane Proteins/metabolism Qa-SNARE Proteins Qb-SNARE Proteins Qc-SNARE Proteins R-SNARE Proteins Rats Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular Transport Proteins
Chemicals
Autoantigens BET1 protein, rat BET1L protein, human Bet1l protein, rat Carrier Proteins Detergents GOSR1 protein, human GOSR2 protein, human Golgi Matrix Proteins Golgin subfamily A member 2 Gosr1 protein, mouse Gosr1 protein, rat Gosr2 protein, rat Membrane Proteins Qa-SNARE Proteins Qb-SNARE Proteins Qc-SNARE Proteins R-SNARE Proteins SEC22A protein, human Sec22a protein, rat Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular Transport Proteins Ykt6 protein, rat macrogolgin vesicular transport factor p115
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shorter James
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520, USA.
Beard Matthew B
Seemann Joachim
Dirac-Svejstrup A Barbara
Warren Graham
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200112127
Published
2002-04-01
Epub
2002-00-01
Pages
45-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173270
Subset
IM
Grants
NIGMS NIH HHS · R01 GM060478 · United States
NIGMS NIH HHS · GM60478-01 · United States
Corrections
CommentIn
PMID: 11970884
External Links
PubMed source DOI: 10.1083/jcb.200112127 PMC Full Text View journal details
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