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PMID: 12045185 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii.

The Journal of cell biology ·Vol. 157 ·No. 6 ·2002-06-10 ·Pages 953-62

Auchincloss AH, Zerges W, Perron K, Girard-Bascou J, Rochaix JD

Abstract

Genetic analysis has revealed that the three nucleus-encoded factors Tbc1, Tbc2, and Tbc3 are involved in the translation of the chloroplast psbC mRNA of the eukaryotic green alga Chlamydomonas reinhardtii. In this study we report the isolation and phenotypic characterization of two new tbc2 mutant alleles and their use for cloning and characterizing the Tbc2 gene by genomic complementation. TBC2 encodes a protein of 1,115 residues containing nine copies of a novel degenerate 38-40 amino acid repeat with a quasiconserved PPPEW motif near its COOH-terminal end. The middle part of the Tbc2 protein displays partial amino acid sequence identity with Crp1, a protein from Zea mays that is implicated in the processing and translation of the chloroplast petA and petD RNAs. The Tbc2 protein is enriched in chloroplast stromal subfractions and is associated with a 400-kD protein complex that appears to play a role in the translation of specifically the psbC mRNA.

MeSH Terms
Alleles Amino Acid Motifs Amino Acid Sequence Animals CCAAT-Enhancer-Binding Proteins/chemistry Cell Nucleus/metabolism Chlamydomonas reinhardtii/genetics,metabolism Chloroplasts/metabolism Cloning, Molecular Conserved Sequence Molecular Sequence Data Mutation Nuclear Proteins/chemistry,genetics,metabolism,physiology Photosynthetic Reaction Center Complex Proteins/genetics Plant Proteins/genetics,metabolism Protein Biosynthesis Protozoan Proteins/chemistry,physiology RNA, Messenger/metabolism Sequence Homology, Amino Acid
Chemicals
CCAAT-Enhancer-Binding Proteins Nuclear Proteins Photosynthetic Reaction Center Complex Proteins Plant Proteins Protozoan Proteins RNA, Messenger tbc2 protein, Chlamydomonas reinhardtii
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Auchincloss Andrea H
Department of Molecular Biology and Department of Plant Biology, University of Geneva, 1211 Geneva 4, Switzerland.
Zerges William
Perron Karl
Girard-Bascou Jacqueline
Rochaix Jean-David
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200201060
Published
2002-06-10
Epub
2002-00-03
Pages
953-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2174033
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200201060 PMC Full Text View journal details
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