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PMID: 9482869 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A poly(A) binding protein functions in the chloroplast as a message-specific translation factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 5 ·1998-03-03 ·Pages 2238-43

Yohn CB, Cohen A, Danon A, Mayfield SP

Abstract

High-affinity binding of a set of proteins with specificity for the 5' untranslated region (UTR) of the Chlamydomonas reinhardtii chloroplast psbA mRNA correlates with light-regulated translational activation of this message. We have isolated a cDNA encoding the main psbA RNA binding protein, RB47, and identified this protein as a member of the poly(A) binding protein family. Poly(A) binding proteins are a family of eukaryotic, cytoplasmic proteins thought to bind poly(A) tails of mRNAs and play a role in translational regulation. In vitro translation of RNA transcribed from the RB47 cDNA produces a precursor protein that is efficiently transported into the chloroplast and processed to the mature 47-kDa protein. RB47 expressed and purified from Escherichia coli binds to the psbA 5' UTR with similar specificity and affinity as RB47 isolated from C. reinhardtii chloroplasts. The identification of a normally cytoplasmic translation factor in the chloroplast suggests that the prokaryotic-like chloroplast translation machinery utilizes a eukaryotic-like initiation factor to regulate the translation of a key chloroplast mRNA. These data also suggest that poly(A) binding proteins may play a wider role in translation regulation than previously appreciated.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Chlamydomonas reinhardtii/metabolism Chloroplasts/metabolism Cloning, Molecular Conserved Sequence Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins/biosynthesis,genetics Photosystem II Protein Complex Poly(A)-Binding Proteins Protein Biosynthesis RNA, Messenger/metabolism RNA-Binding Proteins/biosynthesis,chemistry,metabolism Receptors, Cytoplasmic and Nuclear/metabolism Recombinant Proteins/biosynthesis,chemistry,metabolism Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Poly(A)-Binding Proteins RNA, Messenger RNA-Binding Proteins Receptors, Cytoplasmic and Nuclear Recombinant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yohn C B
Department of Cell Biology and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cohen A
Danon A
Mayfield S P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.5.2238
Published
1998-03-03
Pages
2238-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19305
Subset
IM
Grants
NIGMS NIH HHS · F32 GM015639 · United States
NIGMS NIH HHS · GM15639-03 · United States
Databases
GENBANK
AF043297
External Links
PubMed source DOI: 10.1073/pnas.95.5.2238 PMC Full Text View journal details
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