Home LiteratureArticle Details
PMID: 2761544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of the poly(A)-binding protein during development of Xenopus laevis.

Molecular and cellular biology ·Vol. 9 ·No. 6 ·1989-06-00 ·Pages 2756-60

Zelus BD, Giebelhaus DH, Eib DW, Kenner KA, Moon RT

Abstract

We have isolated and sequenced cDNA clones encoding the poly(A)-binding protein of Xenopus laevis oocytes. Polyclonal antiserum was raised against a fusion protein encoding 185 amino acids of the Xenopus poly(A)-binding protein. This antiserum localizes the poly(A)-binding protein to subcellular sites associated with protein synthesis; in the retina, immunoreactive protein is detected in the synthetically active inner segment of the photoreceptor but not in the transductive outer segment. Transcripts encoding the poly(A)-binding protein are present in oocytes, although no protein is detected on protein blots. In contrast, the levels of both transcripts and protein increase in development, which correlates with the observed increase in total poly(A) during Xenopus embryogenesis (N. Sagata, K. Shiokawa, and K. Yamana, Dev. Biol. 77:431-448, 1980).

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/biosynthesis,genetics Cell Differentiation Codon DNA/genetics DNA Probes Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation Molecular Sequence Data Oocytes/cytology Poly A/biosynthesis,metabolism Poly(A)-Binding Proteins RNA, Messenger/biosynthesis Rabbits Retina/cytology Xenopus laevis/embryology,genetics
Chemicals
Carrier Proteins Codon DNA Probes Poly(A)-Binding Proteins RNA, Messenger Poly A DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zelus B D
Department of Pharmacology, School of Medicine, University of Washington, Seattle 98915.
Giebelhaus D H
Eib D W
Kenner K A
Moon R T
References (29)
29 references, click to expand
  1. A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):924-8 PMID: 4515002
  2. CHANGES IN DNA, RNA, AND PROTEIN SYNTHESIS IN THE DEVELOPING LENS.
    Invest Ophthalmol. 1965 Aug;4:480-95 PMID: 14340163
  3. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  4. A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
    Dev Biol. 1980 Jun 15;77(2):431-48 PMID: 6156874
  5. Translational stability of native and deadenylylated rabbit globin mRNA injected into HeLa cells.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):908-11 PMID: 6940155
  6. Proteins associated with poly(A) and other regions of mRNA and hnRNA molecules as investigated by crosslinking.
    Cell. 1981 Jun;24(3):775-83 PMID: 7249081
  7. Determination of nucleotide sequences in DNA.
    Science. 1981 Dec 11;214(4526):1205-10 PMID: 7302589
  8. Newly formed mRNA lacking polyadenylic acid enters the cytoplasm and the polyribosomes but has a shorter half-life in the absence of polyadenylic acid.
    Mol Cell Biol. 1982 May;2(5):517-25 PMID: 6981059
  9. The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein.
    J Cell Biol. 1983 Mar;96(3):717-21 PMID: 6833379
  10. The use of Xenopus oocytes for the expression of cloned genes.
    Methods Enzymol. 1983;101:370-86 PMID: 6193395
  11. Possible involvement of poly(A) in protein synthesis.
    Nucleic Acids Res. 1983 Sep 24;11(18):6353-68 PMID: 6137807
  12. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  13. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  14. Tissue-specific expression of distinct spectrin and ankyrin transcripts in erythroid and nonerythroid cells.
    J Cell Biol. 1985 Jan;100(1):152-60 PMID: 2981230
  15. Identification and cloning of localized maternal RNAs from Xenopus eggs.
    Cell. 1985 Oct;42(3):769-77 PMID: 2414011
  16. The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7375-94 PMID: 3932972
  17. A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
    Cell. 1986 Jun 20;45(6):827-35 PMID: 3518950
  18. mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
    Mol Cell Biol. 1986 Aug;6(8):2932-43 PMID: 3537727
  19. The function of proteins that interact with mRNA.
    Mol Cell Biochem. 1987 Mar;74(1):5-15 PMID: 3295516
  20. Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.
    Mol Cell Biol. 1987 May;7(5):1731-9 PMID: 3110598
  21. Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
    Nucleic Acids Res. 1987 Jun 25;15(12):4771-87 PMID: 2885805
  22. Changes in the expression of alpha-fodrin during embryonic development of Xenopus laevis.
    J Cell Biol. 1987 Aug;105(2):843-53 PMID: 3040772
  23. Role of the 3'-poly(A) sequence in translational regulation of mRNAs in Xenopus laevis oocytes.
    J Biol Chem. 1988 Apr 25;263(12):5764-70 PMID: 3356706
  24. Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus.
    Cell. 1988 May 20;53(4):601-15 PMID: 2453290
  25. Microinjection of synthetic Xhox-1A homeobox mRNA disrupts somite formation in developing Xenopus embryos.
    Cell. 1988 Jun 3;53(5):687-97 PMID: 2897242
  26. Determinants of mRNA stability in Dictyostelium discoideum amoebae: differences in poly(A) tail length, ribosome loading, and mRNA size cannot account for the heterogeneity of mRNA decay rates.
    Mol Cell Biol. 1988 May;8(5):1957-69 PMID: 2898728
  27. Photocrosslinking of proteins to maternal mRNA in Xenopus oocytes.
    Dev Biol. 1988 Aug;128(2):349-58 PMID: 2456235
  28. The poly(A)-binding protein facilitates in vitro translation of poly(A)-rich mRNA.
    Eur J Biochem. 1988 Oct 1;176(3):521-6 PMID: 2901956
  29. The translational capacity of deadenylated ovalbumin messenger RNA.
    Cell. 1976 May;8(1):51-8 PMID: 954092
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.6.2756-2760.1989
Published
1989-06-00
Pages
2756-60
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362352
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GM07270 · United States
NIDDK NIH HHS · DK35774 · United States
Databases
GENBANK
M27072
External Links
PubMed source DOI: 10.1128/mcb.9.6.2756-2760.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2