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PMID: 12189553 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A model for co-expression pattern analysis of genes implicated in angiogenesis and tumour cell invasion in cervical cancer.

British journal of cancer ·Vol. 87 ·No. 5 ·2002-08-27 ·Pages 537-44

Van Trappen PO, Ryan A, Carroll M, Lecoeur C, Goff L, Gyselman VG, Young BD, Lowe DG, Pepper MS, Shepherd JH, Jacobs IJ

Abstract

To date, numerous genes have been identified which are involved in both tumour neovascularisation (angiogenesis) and tumour cell invasion, and most of them are also expressed to some extent under normal physiological conditions. However, little is known about how these genes co-express in these settings. This study was undertaken to quantitate mRNA levels in normal and malignant cervical tissues of nine selected genes (VEGF(121), VEGF(165), VEGF(189), VEGF-C, eIF-4E, b-FGF, TSP-2, MMP-2 and MMP-9) implicated in the above processes using real-time quantitative RT-PCR. In addition, the Spearman's rank correlation was used to determine their co-expression patterns. The transcript levels for the different VEGF-A splice variants (VEGF(121), VEGF(165), VEGF(189)) were at least 10-fold higher in the cancer cases, with the highest levels in the primary tumours demonstrating lympho-vascular space involvement. The lymphangiogenic factor VEGF-C and MMP-9 were upregulated 130- and 80-fold respectively in cervical cancers. The highest levels of VEGF-C mRNA were found in the lymph-node positive group. The transcript levels for b-FGF were similar in normal cervical tissue and early-stage cervical cancer, however, higher levels were found in the cervical cancers with advanced stage disease. Comparing gene transcript levels between recurrent and non-recurrent cervical cancer patients revealed significant differences (P=0.038) in transcript levels for the angiogenesis inhibitor TSP-2, with the highest levels in non-recurrent cases. Co-expression pattern analysis in normal cervical tissue revealed highly significant co-expressions (P<0.0001) between TSP-2 and most other genes analysed (VEGF(121), VEGF(165), VEGF-C, b-FGF and MMP-2). In cervical cancer, TSP-2 appears only to be highly co-expressed with MMP-2 (P<0.0001). In contrast to normal cervical tissue, we found a highly significant co-expression (P<0.0001) between MMP-9 and VEGF(189) in cervical cancer. The combined application of real-time quantitative RT-PCR and Spearman's rank correlation identifies gene transcripts which are simultaneously co-expressed. Our results revealed a significant co-expression between the angiogenesis inhibitor TSP-2 and most other genes analysed in normal cervical tissue. In cervical cancer, we found a strong upregulation of VEGF-C and MMP-9 mRNA, with a highly significant co-expression between MMP-9 and VEGF(189).

MeSH Terms
Cervix Uteri/metabolism Endothelial Growth Factors/biosynthesis,genetics Eukaryotic Initiation Factor-4E Female Fibroblast Growth Factors/biosynthesis,genetics Gene Expression Regulation, Neoplastic Humans Lymphokines/biosynthesis,genetics Matrix Metalloproteinase 2/biosynthesis,genetics Matrix Metalloproteinase 9/biosynthesis,genetics Models, Genetic Neoplasm Invasiveness/genetics Neoplasm Proteins/biosynthesis,genetics Neoplasm Recurrence, Local Neovascularization, Pathologic/genetics Neovascularization, Physiologic/genetics Peptide Initiation Factors/biosynthesis,genetics Protein Isoforms/biosynthesis,genetics RNA, Messenger/biosynthesis RNA, Ribosomal, 18S/biosynthesis,genetics Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic Uterine Cervical Neoplasms/genetics,metabolism Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors
Chemicals
Endothelial Growth Factors Eukaryotic Initiation Factor-4E Lymphokines Neoplasm Proteins Peptide Initiation Factors Protein Isoforms RNA, Messenger RNA, Ribosomal, 18S VEGFA protein, human Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors Fibroblast Growth Factors Matrix Metalloproteinase 2 Matrix Metalloproteinase 9
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Van Trappen P O
Department of Gynaecological Oncology, Cancer Research UK Translational Oncology Laboratory, John Vane Science Centre, Queen Mary University of London, London, UK. [email protected]
Ryan A
Carroll M
Lecoeur C
Goff L
Gyselman V G
Young B D
Lowe D G
Pepper M S
Shepherd J H
Jacobs I J
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/sj.bjc.6600471
Email
[email protected]
Published
2002-08-27
Pages
537-44
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2376148
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.bjc.6600471 PMC Full Text View journal details
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