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PMID: 12200547 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 19 ·2002-09-17 ·Pages 12391-6

Tettelin H, Masignani V, Cieslewicz MJ, Eisen JA, Peterson S, Wessels MR, Paulsen IT, Nelson KE, Margarit I, Read TD, Madoff LC, Wolf AM, Beanan MJ, Brinkac LM, Daugherty SC, DeBoy RT, Durkin AS, Kolonay JF, Madupu R, Lewis MR, Radune D, Fedorova NB, Scanlan D, Khouri H, Mulligan S, Carty HA, Cline RT, Van Aken SE, Gill J, Scarselli M, Mora M, Iacobini ET, Brettoni C, Galli G, Mariani M, Vegni F, Maione D, Rinaudo D, Rappuoli R, Telford JL, Kasper DL, Grandi G, Fraser CM

Abstract

The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.

MeSH Terms
Amino Acid Sequence Biological Evolution Genome, Bacterial Humans Molecular Sequence Data Oligonucleotide Array Sequence Analysis Phylogeny Serotyping Species Specificity Streptococcal Infections/microbiology Streptococcus agalactiae/classification,genetics,pathogenicity Streptococcus pneumoniae/genetics Streptococcus pyogenes/genetics Virulence/genetics
Authors & Affiliations
43 authors, click to expand affiliations / ORCID
Tettelin Herve
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
Masignani Vega
Cieslewicz Michael J
Eisen Jonathan A
Peterson Scott
Wessels Michael R
Paulsen Ian T
Nelson Karen E
Margarit Immaculada
Read Timothy D
Madoff Lawrence C
Wolf Alex M
Beanan Maureen J
Brinkac Lauren M
Daugherty Sean C
DeBoy Robert T
Durkin A Scott
Kolonay James F
Madupu Ramana
Lewis Matthew R
Radune Diana
Fedorova Nadezhda B
Scanlan David
Khouri Hoda
Mulligan Stephanie
Carty Heather A
Cline Robin T
Van Aken Susan E
Gill John
Scarselli Maria
Mora Marirosa
Iacobini Emilia T
Brettoni Cecilia
Galli Giuliano
Mariani Massimo
Vegni Filippo
Maione Domenico
Rinaudo Daniela
Rappuoli Rino
Telford John L
Kasper Dennis L
Grandi Guido
Fraser Claire M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.182380799
Published
2002-09-17
Epub
2002-00-28
Pages
12391-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC129455
Subset
IM
Grants
NIAID NIH HHS · R56 AI038424 · United States
NIAID NIH HHS · AI75326 · United States
NIAID NIH HHS · R21 AI042940 · United States
NIAID NIH HHS · AI23339 · United States
NIAID NIH HHS · R15 AI075326 · United States
NIAID NIH HHS · R01 AI042940 · United States
NIAID NIH HHS · AI38424 · United States
NIAID NIH HHS · AI42940 · United States
NIAID NIH HHS · R01 AI038424 · United States
Databases
GENBANK
AE009948
External Links
PubMed source DOI: 10.1073/pnas.182380799 PMC Full Text View journal details
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