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PMID: 12239413 Published · ppublish English Journal Article

A Single Gene May Encode Differentially Localized Ca2+-ATPases in Tomato.

The Plant cell ·Vol. 8 ·No. 7 ·1996-07-00 ·Pages 1159-1169

Ferrol N, Bennett AB

Abstract

Previously, a partial-length cDNA and a complete genomic clone encoding a putative sarcoplasmic reticulum-type Ca2+-ATPase (LCA, Lycopersicon Ca2+-ATPase) were isolated from tomato. To determine the subcellular localization of this Ca2+-ATPase, specific polyclonal antibodies raised against a fusion protein encoding a portion of the LCA polypeptide were generated. Based on hybridization of the LCA cDNA and of the nucleotide sequence encoding the fusion protein to genomic DNA, it appears that LCA and the fusion protein domain are encoded by a single gene in tomato. Antibodies raised against the LCA domain fusion protein reacted specifically with two polypeptides of 116 and 120 kD that are localized in the vacuolar and plasma membranes, respectively. The distribution of vanadate-sensitive ATP-dependent Ca2+ transport activities in sucrose gradients coincided with the distribution of the immunodetected proteins. The ATP-dependent Ca2+ transport activities associated with tonoplast and plasma membrane fractions shared similar properties, because both fractions were inhibited by vanadate but insensitive to carbonyl cyanide m-chlorophenylhydrazone, nitrate, and calmodulin. Moreover, antibodies raised against the LCA domain fusion protein inhibited ATP-dependent Ca2+ uptake activity associated with both the tonoplast and plasma membrane fractions. These data suggest that a single gene (LCA) may encode two P-type Ca2+-ATPase isoforms that are differentially localized in the tonoplast and plasma membrane of tomato roots.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferrol N.
Mann Laboratory, Department of Vegetable Crops, University of California at Davis, Davis, California 95616.
Bennett A. B.
References (34)
34 references, click to expand
  1. Use of monoclonal antibodies to analyse the expression of a multi-tubulin family.
    FEBS Lett. 1985 Aug 5;187(2):211-8 PMID: 4018260
  2. Calcium pump of the plasma membrane.
    Physiol Rev. 1991 Jan;71(1):129-53 PMID: 1986387
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Calcium-pumping ATPases in vesicles from carrot cells : stimulation by calmodulin or phosphatidylserine, and formation of a 120 kilodalton phosphoenzyme.
    Plant Physiol. 1991 Dec;97(4):1535-44 PMID: 16668581
  5. Investigation of the Calcium-Transporting ATPases at the Endoplasmic Reticulum and Plasma Membrane of Red Beet (Beta vulgaris).
    Plant Physiol. 1993 Jun;102(2):553-564 PMID: 12231844
  6. Calcium Fluxes across the Plasma Membrane of Commelina communis L. Assayed in a Cell-Free System.
    Plant Physiol. 1990 Jul;93(3):940-7 PMID: 16667604
  7. The KKXX signal mediates retrieval of membrane proteins from the Golgi to the ER in yeast.
    Eur J Cell Biol. 1994 Jun;64(1):211-6 PMID: 7957309
  8. A signal for endoplasmic reticulum retention located at the carboxyl terminus of the plasma membrane Ca(2+)-ATPase isoform 4CI.
    J Biol Chem. 1995 Feb 10;270(6):2679-88 PMID: 7852337
  9. Further Characterization of the Red Beet Plasma Membrane Ca-ATPase Using GTP as an Alternative Substrate.
    Plant Physiol. 1990 Mar;92(3):747-54 PMID: 16667344
  10. Transport Properties of the Tomato Fruit Tonoplast : III. Temperature Dependence of Calcium Transport.
    Plant Physiol. 1988 Dec;88(4):1097-103 PMID: 16666428
  11. Regulation of splicing is responsible for the expression of the muscle-specific 2a isoform of the sarco/endoplasmic-reticulum Ca(2+)-ATPase.
    Biochem J. 1994 Sep 1;302 ( Pt 2):559-66 PMID: 7522437
  12. Defense strategies against hypoxia and hypothermia.
    Science. 1986 Jan 17;231(4735):234-41 PMID: 2417316
  13. Calcium and proton transport in membrane vesicles from barley roots.
    Plant Physiol. 1990 Sep;94(1):179-88 PMID: 16667684
  14. The calmodulin-stimulated ATPase of maize coleoptiles forms a phosphorylated intermediate.
    Biochem Biophys Res Commun. 1989 Feb 28;159(1):185-91 PMID: 2522300
  15. Induction of a Putative Ca-ATPase mRNA in NaCl-Adapted Cells.
    Plant Physiol. 1992 Nov;100(3):1471-8 PMID: 16653146
  16. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  17. The endoplasmic reticulum retention signal of the E3/19K protein of adenovirus type 2 consists of three separate amino acid segments at the carboxy terminus.
    J Cell Biol. 1990 Nov;111(5 Pt 1):1803-10 PMID: 2146274
  18. Reconstitution and Characterization of a Calmodulin-Stimulated Ca-Pumping ATPase Purified from Brassica oleracea L.
    Plant Physiol. 1992 Dec;100(4):1670-81 PMID: 16653183
  19. Ca-translocating ATPase of the plant plasma membrane.
    Plant Physiol. 1990 Oct;94(2):397-400 PMID: 16667727
  20. Plasma Membrane Ca-ATPase of Radish Seedlings : II. Regulation by Calmodulin.
    Plant Physiol. 1992 Mar;98(3):1202-6 PMID: 16668747
  21. Control of protein exit from the endoplasmic reticulum.
    Annu Rev Cell Biol. 1989;5:1-23 PMID: 2688704
  22. Calcium transport in tonoplast and endoplasmic reticulum vesicles isolated from cultured carrot cells.
    Plant Physiol. 1986 Feb;80(2):549-55 PMID: 16664660
  23. Calcium and signal transduction in plants.
    CRC Crit Rev Plant Sci. 1993;12(3):185-211 PMID: 11540065
  24. Mechanism of aluminum inhibition of net ca uptake by amaranthus protoplasts.
    Plant Physiol. 1992 Feb;98(2):632-8 PMID: 16668688
  25. The calcium pump of the surface membrane and of the sarcoplasmic reticulum.
    Annu Rev Physiol. 1989;51:473-85 PMID: 2540700
  26. Molecular cloning of cDNAs from human kidney coding for two alternatively spliced products of the cardiac Ca2+-ATPase gene.
    J Biol Chem. 1988 Oct 15;263(29):15024-31 PMID: 2844796
  27. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  28. Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum.
    J Cell Sci. 1992 Jun;102 ( Pt 2):261-71 PMID: 1383243
  29. Higher plant Ca(2+)-ATPase: primary structure and regulation of mRNA abundance by salt.
    Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9205-9 PMID: 1384045
  30. Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.
    EMBO J. 1990 Oct;9(10):3153-62 PMID: 2120038
  31. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  32. Controlled Proteolysis Activates the Plasma Membrane Ca2+ Pump of Higher Plants (A Comparison with the Effect of Calmodulin in Plasma Membrane from Radish Seedlings).
    Plant Physiol. 1993 Oct;103(2):385-390 PMID: 12231945
  33. Ca2+ pump and Ca2+/H+ antiporter in plasma membrane vesicles isolated by aqueous two-phase partitioning from corn leaves.
    J Membr Biol. 1990 Mar;114(2):133-42 PMID: 2160540
  34. The sarcoplasmic reticulum Ca(2+)-ATPase, SERCA1a, contains endoplasmic reticulum targeting information.
    Biochem Biophys Res Commun. 1992 Jul 15;186(1):219-27 PMID: 1385945
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.8.7.1159
Published
1996-07-00
Pages
1159-1169
Language
English
Region
England
NLM ID
9208688
PMCID
PMC161195
External Links
PubMed source DOI: 10.1105/tpc.8.7.1159 PMC Full Text View journal details
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