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PMID: 2146274 Published · ppublish English Journal Article

The endoplasmic reticulum retention signal of the E3/19K protein of adenovirus type 2 consists of three separate amino acid segments at the carboxy terminus.

The Journal of cell biology ·Vol. 111 ·No. 5 Pt 1 ·1990-11-00 ·Pages 1803-10

Gabathuler R, Kvist S

Abstract

The E3/19K protein of adenovirus type 2 is a resident of the ER. Immediately after synthesis it binds to human major histocompatibility complex class I antigens and prevents their departure from the ER compartment. The ER retention signal of the E3/19K protein is contained within the 15 amino acids that protrude on the cytoplasmic side at the carboxy terminus of the protein. To define the ER retention sequence in more detail, we have generated 10 mutants of the E3/19K protein that differ only within this segment. Analysis of the rate of intracellular transport and cell surface expression of HLA antigens associated to these mutants, show that the sequences Ser-Phe-Ile, located in the middle of the 15-residue segment and Met-Pro, at the extreme carboxy terminus, are crucial for retention. Four charged residues, Asp-Glu-Lys-Lys, are located between these two retention elements but are of little or no importance. The basic cluster of amino acids close to the membrane also has some effect on retention. Thus, the retention signal of the E3/19K protein is not a contiguous sequence of amino acids but has a complex spatial arrangement.

MeSH Terms
Adenovirus Early Proteins Amino Acid Sequence Antigens, Surface/metabolism Cell Line Endoplasmic Reticulum/metabolism HLA Antigens/metabolism Humans Molecular Sequence Data Mutagenesis, Site-Directed Oncogene Proteins, Viral/chemistry,genetics,metabolism Protein Sorting Signals/chemistry Structure-Activity Relationship Transfection
Chemicals
Adenovirus Early Proteins Antigens, Surface HLA Antigens Oncogene Proteins, Viral Protein Sorting Signals
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gabathuler R
Ludwig Institute for Cancer Research, Stockholm, Sweden.
Kvist S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.5.1803
Published
1990-11-00
Pages
1803-10
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116308
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.5.1803 PMC Full Text View journal details
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