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PMID: 6985478 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple mRNA species for the precursor to an adenovirus-encoded glycoprotein: identification and structure of the signal sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6349-53

Persson H, Jörnvall H, Zabielski J

Abstract

Early region 3 of the adenovirus type 2 genome encodes three proteins with molecular weights of 16,000, 14,500, and 14,000 (E2/16, E3/14.5, and E3/14). The E3/16 protein is the precursor to the E3/19 glycoprotein and is around 1500 daltons larger than the unglycosylated E3/19O protein. The E3/14.5 and E3/14 proteins are structurally related to each other but different from E3/16. Three mRNA species were identified for E3/16; all have common 5' ends with the same spliced region but with different 3' ends. E3/14 was translated from a 13S mRNA with the same 5' structure as the E3/16 mRNA but followed by a second spliced region with a different 3' end. A partial amino acid sequence was determined for E3/16 after radioactive labeling in vitro and this sequence can be aligned with a known DNA sequence. It contains a hydrophobic signal sequence, two presumptive glycosylation sites, and a hydrophobic region close to the COOH terminus.

MeSH Terms
Adenoviruses, Human/genetics Amino Acid Sequence Base Sequence DNA, Viral/genetics Genes, Viral Glycoproteins/genetics Protein Precursors/genetics RNA, Messenger/genetics RNA, Viral/genetics Viral Proteins/genetics
Chemicals
DNA, Viral Glycoproteins Protein Precursors RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Persson H
Jörnvall H
Zabielski J
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6349
Published
1980-11-00
Pages
6349-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350281
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1073/pnas.77.11.6349 PMC Full Text View journal details
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