Home LiteratureArticle Details
PMID: 2958275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The E3/19K protein of adenovirus type 2 binds to the domains of histocompatibility antigens required for CTL recognition.

The EMBO journal ·Vol. 6 ·No. 7 ·1987-07-00 ·Pages 2019-26

Burgert HG, Kvist S

Abstract

The E3/19K protein of human adenovirus type 2 binds to HLA class I antigens and blocks their terminal glycosylation and cell surface expression. The nature of this interaction is non-covalent and involves neither disulfide bridges between the two molecules nor their carbohydrates. The murine H-2 Kd antigen associates with the E3/19K protein in a similar fashion to human HLA antigens whereas the allelic product H-2 Kk does not. Hybrid genes between the Kd and Kk alleles were constructed and their products were expressed in embryonic kidney cells together with the E3/19K protein. This allowed us to identify the alpha 1 and alpha 2 domains as the essential structures of the histocompatibility antigens for binding the viral protein. Interestingly, these domains are also crucial for T cell recognition. The implications for the evolution of adenoviruses and their ability to cause persistent infections are discussed.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics,immunology Animals Antigens, Viral, Tumor/immunology Biological Evolution Cell Line Cytotoxicity, Immunologic H-2 Antigens/genetics,immunology HLA Antigens/immunology Oncogene Proteins, Viral/immunology
Chemicals
Adenovirus Early Proteins Antigens, Viral, Tumor H-2 Antigens HLA Antigens Oncogene Proteins, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burgert H G
Swiss Institute for Experimental Cancer Research, Epalinges s/Lausanne, Switzerland.
Kvist S
References (47)
47 references, click to expand
  1. Different structural constraints for recognition of mouse H-2Kd and -Kk antigens by alloimmune cytolytic T lymphocytes.
    J Exp Med. 1986 Dec 1;164(6):1823-34 PMID: 3491173
  2. Structural and functional dissection of an MHC class I antigen-binding adenovirus glycoprotein.
    EMBO J. 1986 Aug;5(8):1921-7 PMID: 3019670
  3. The genomic map position of the adenovirus type 2 glycoprotein.
    Virology. 1979 Dec;99(2):427-30 PMID: 516454
  4. Complex splicing patterns of RNAs from the early regions of adenovirus-2.
    J Mol Biol. 1979 Oct 25;134(2):265-303 PMID: 537065
  5. Nucleotide sequence of the EcoRI D fragment of adenovirus 2 genome.
    Nucleic Acids Res. 1980 May 24;8(10):2173-92 PMID: 6253880
  6. Multiple mRNA species for the precursor to an adenovirus-encoded glycoprotein: identification and structure of the signal sequence.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6349-53 PMID: 6985478
  7. A subcloning strategy for DNA sequence analysis.
    Nucleic Acids Res. 1980 Dec 11;8(23):5541-9 PMID: 6258146
  8. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
    Cell. 1981 May;24(2):287-99 PMID: 7016338
  9. Structures of the oligosaccharides of the glycoprotein coded by early region E3 of adenovirus 2.
    J Virol. 1981 Nov;40(2):440-9 PMID: 7321093
  10. Regulation of adenovirus gene expression.
    Curr Top Microbiol Immunol. 1982;97:157-203 PMID: 7035073
  11. Membrane insertion and oligomeric assembly of HLA-DR histocompatibility antigens.
    Cell. 1982 May;29(1):61-9 PMID: 6955026
  12. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  13. An adenovirus glycoprotein binds heavy chains of class I transplantation antigens from man and mouse.
    Nature. 1982 Sep 9;299(5879):175-8 PMID: 6287288
  14. The nucleotide sequence of mRNA for the Mr 19 000 glycoprotein from early gene block III of adenovirus 2.
    Gene. 1982 Dec;20(3):339-46 PMID: 6985487
  15. Allospecific and virus-specific cytolytic T lymphocytes are restricted to the N or C1 domain of H-2 antigens expressed on L cells after DNA-mediated gene transfer.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2709-12 PMID: 6302702
  16. Association between transplantation antigens and a viral membrane protein synthesized from a mammalian expression vector.
    Cell. 1983 Jun;33(2):445-53 PMID: 6305511
  17. Complex formation of class I transplantation antigens and a viral glycoprotein.
    J Biol Chem. 1983 Sep 10;258(17):10594-8 PMID: 6885796
  18. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites.
    Nature. 1984 May 17-23;309(5965):279-81 PMID: 6201750
  19. Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4381-5 PMID: 6336330
  20. Cytolytic T cells recognize the two amino-terminal domains of H-2 K antigens in tandem in influenza A infected cells.
    Cell. 1984 Aug;38(1):79-87 PMID: 6088078
  21. Genetic analysis of adenoviruses.
    Curr Top Microbiol Immunol. 1984;111:1-39 PMID: 6488878
  22. Complete nucleotide sequence of the murine H-2Kk gene. Comparison of three H-2K locus alleles.
    Nucleic Acids Res. 1984 Dec 21;12(24):9473-87 PMID: 6096831
  23. The 19-kDa glycoprotein coded by region E3 of adenovirus. Purification, characterization, and structural analysis.
    J Biol Chem. 1985 Feb 25;260(4):2424-31 PMID: 3882694
  24. Construction of a fusion gene that confers resistance against hygromycin B to mammalian cells in culture.
    Exp Cell Res. 1985 May;158(1):237-43 PMID: 2987009
  25. An adenovirus type 2 glycoprotein blocks cell surface expression of human histocompatibility class I antigens.
    Cell. 1985 Jul;41(3):987-97 PMID: 3924414
  26. The polypeptides of adenovirus. VII. Further studies of early polypeptides in vivo and localization of E2 and E2A to the cell plasma membrane.
    Virology. 1976 Jun;71(2):518-30 PMID: 936473
  27. Mouse histocompatibility genes: structure and organisation of a Kd gene.
    EMBO J. 1983;2(2):245-54 PMID: 11894934
  28. Intracellular transport of membrane glycoproteins: two closely related histocompatibility antigens differ in their rates of transit to the cell surface.
    J Cell Biol. 1985 Sep;101(3):725-34 PMID: 3928633
  29. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  30. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 1978 May;14(1):9-20 PMID: 667938
  31. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  32. Molecular association between transplantation antigens and cell surface antigen in adenovirus-transformed cell line.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5674-8 PMID: 310123
  33. The major histocompatibility complex of the mouse.
    Science. 1979 Feb 9;203(4380):516-21 PMID: 104386
  34. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness.
    Adv Immunol. 1979;27:51-177 PMID: 92183
  35. Alloreactive cytolytic T-cell clones preferentially recognize conformational determinants on histocompatibility antigens: analysis with genetically engineered hybrid antigens.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):7030-4 PMID: 2413454
  36. Mutations that impair a posttranscriptional step in expression of HLA-A and -B antigens.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8183-7 PMID: 3906658
  37. Impaired intracellular transport of class I MHC antigens as a possible means for adenoviruses to evade immune surveillance.
    Cell. 1985 Nov;43(1):215-22 PMID: 2934137
  38. Class I genes and molecules: an update.
    Immunology. 1986 Jan;57(1):3-18 PMID: 2417940
  39. Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):757-61 PMID: 3080749
  40. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides.
    Cell. 1986 Mar 28;44(6):959-68 PMID: 2420472
  41. Murine major histocompatibility complex class-I mutants: molecular analysis and structure-function implications.
    Annu Rev Immunol. 1986;4:471-502 PMID: 3518748
  42. Impaired assembly and transport of HLA-A and -B antigens in a mutant TxB cell hybrid.
    EMBO J. 1986 May;5(5):943-9 PMID: 3522223
  43. Differential association between two human MHC class I antigens and an adenoviral glycoprotein.
    J Immunol. 1986 Aug 1;137(3):1003-9 PMID: 2941483
  44. Genetic analysis of mRNA synthesis in adenovirus region E3 at different stages of productive infection by RNA-processing mutants.
    J Virol. 1986 Oct;60(1):54-63 PMID: 3747028
  45. Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface.
    Cell. 1986 Sep 12;46(6):929-37 PMID: 3019557
  46. Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport.
    Cell. 1986 Sep 12;46(6):939-50 PMID: 3757030
  47. "E3/19K" protein of adenovirus type 2 inhibits lysis of cytolytic T lymphocytes by blocking cell-surface expression of histocompatibility class I antigens.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1356-60 PMID: 2950523
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-07-00
Pages
2019-26
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553591
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2