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PMID: 12358428 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ngl2p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae.

RNA (New York, N.Y.) ·Vol. 8 ·No. 9 ·2002-09-00 ·Pages 1095-101

Faber AW, Van Dijk M, Raué HA, Vos JC

Abstract

Saccharomyces cerevisiae contains three nonessential genes (NGL1, NGL2, and NGL3) that encode proteins containing a domain with similarity to a Mg(2+)-dependent endonuclease motif present in the mRNA deadenylase Ccr4p. We have investigated a possible role of these proteins in rRNA processing, because for many of the pre-rRNA processing steps, the identity of the responsible nuclease remains elusive. Analysis of RNA isolated from cells in which the NGL2 gene has been inactivated (ngl2delta) demonstrates that correct 3'-end formation of 5.8S rRNA at site E is strictly dependent on Ngl2p. No role in pre-rRNA processing could be assigned to Ngl1p and Ngl3p. The 3'-extended 5.8S rRNA formed in the ngl2delta mutant is slightly shorter than the 6S precursor previously shown to accumulate upon combined deletion of the 3' --> 5' exonuclease-encoding REX1 and REX2 genes or upon depletion of the exosomal subunits Rrp40p or Rrp45p. Thus, our data add a further component to the set of nucleases required for correct 3'-end formation of yeast 5.8S rRNA.

MeSH Terms
Amino Acid Sequence Base Sequence Gene Deletion Genes, Fungal Models, Molecular Molecular Sequence Data Nucleic Acid Conformation RNA Precursors/chemistry,genetics,metabolism RNA Processing, Post-Transcriptional RNA, Fungal/chemistry,genetics,metabolism RNA, Ribosomal, 5.8S/chemistry,genetics,metabolism Ribonucleases/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism
Chemicals
RNA Precursors RNA, Fungal RNA, Ribosomal, 5.8S Saccharomyces cerevisiae Proteins CCR4 protein, S cerevisiae Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Faber Alex W
Department of Biochemistry and Molecular Biology, Instituut Moleculair Biologische Wetenschappen, Vrije Universiteit, Amsterdam, The Netherlands.
Van Dijk Marie
Raué Hendrik A
Vos Jan C
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838202021027
Published
2002-09-00
Pages
1095-101
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1370323
Subset
IM
External Links
PubMed source DOI: 10.1017/s1355838202021027 PMC Full Text View journal details
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