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PMID: 8290578 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The RNA of RNase MRP is required for normal processing of ribosomal RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 2 ·1994-01-18 ·Pages 659-63

Chu S, Archer RH, Zengel JM, Lindahl L

Abstract

We have isolated clones which complement the temperature sensitivity and abnormal rRNA processing pattern of the rrp2-2 mutant of Saccharomyces cerevisiae we previously described. DNA sequencing and restriction analysis demonstrated that all clones contain the NME1 gene encoding the RNA of the ribonucleprotein particle RNase MRP. Deletion analysis showed that the NME1 gene is responsible for the complementation of the rrp2-2 phenotype. A single base change was identified in the nme1 gene in the rrp2 mutant, confirming that the RRP2 and NME1 genes are identical. Our experiments therefore indicate that RNase MRP, in addition to its previously reported role in formation of RNA primers for mitochondrial DNA replication [Clayton, D. A. (1991) Trends Biochem. Sci. 16, 107-111], is involved in rRNA processing.

Related Genes
NME1 RRP2
MeSH Terms
Base Sequence Cloning, Molecular Endoribonucleases/metabolism Genes, Fungal Genetic Complementation Test Molecular Sequence Data Mutation Nucleic Acid Conformation RNA Processing, Post-Transcriptional/genetics RNA, Fungal/chemistry,genetics,metabolism RNA, Ribosomal/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Temperature
Chemicals
RNA, Fungal RNA, Ribosomal Endoribonucleases mitochondrial RNA-processing endoribonuclease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chu S
Department of Biology, University of Rochester, NY 14627.
Archer R H
Zengel J M
Lindahl L
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.2.659
Published
1994-01-18
Pages
659-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43008
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.2.659 PMC Full Text View journal details
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