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PMID: 12399375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription and double-strand breaks induce similar mitotic recombination events in Saccharomyces cerevisiae.

Genetics ·Vol. 162 ·No. 2 ·2002-10-00 ·Pages 603-14

González-Barrera S, García-Rubio M, Aguilera A

Abstract

We have made a comparative analysis of double-strand-break (DSB)-induced recombination and spontaneous recombination under low- and high-transcription conditions in yeast. We constructed two different recombination substrates, one for the analysis of intermolecular gene conversions and the other for intramolecular gene conversions and inversions. Such substrates were based on the same leu2-HOr allele fused to the tet promoter and containing a 21-bp HO site. Gene conversions and inversions were differently affected by rad1, rad51, rad52, and rad59 single and double mutations, consistent with the actual view that such events occur by different recombination mechanisms. However, the effect of each mutation on each type of recombination event was the same, whether associated with transcription or induced by the HO-mediated DSB. Both the highly transcribed DNA and the HO-cut sequence acted as recipients of the gene conversion events. These results are consistent with the hypothesis that transcription promotes initiation of recombination along the DNA sequence being transcribed. The similarity between transcription-associated and DSB-induced recombination suggests that transcription promotes DNA breaks.

MeSH Terms
Chromosome Inversion DNA Damage DNA Repair Enzymes DNA-Binding Proteins/genetics,metabolism Endonucleases/genetics,metabolism Gene Conversion/physiology Rad51 Recombinase Rad52 DNA Repair and Recombination Protein Recombination, Genetic Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
DNA-Binding Proteins RAD52 protein, S cerevisiae RAD59 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins RAD51 protein, S cerevisiae Rad51 Recombinase Endonucleases RAD1 protein, S cerevisiae DNA Repair Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
González-Barrera Sergio
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
García-Rubio María
Aguilera Andrés
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/162.2.603
Published
2002-10-00
Pages
603-14
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462300
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/162.2.603 PMC Full Text View journal details
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