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PMID: 7705617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of reciprocal exchange, one-ended invasion crossover and single-strand annealing on inverted and direct repeat recombination in yeast: different requirements for the RAD1, RAD10, and RAD52 genes.

Genetics ·Vol. 139 ·No. 1 ·1995-01-00 ·Pages 109-23

Prado F, Aguilera A

Abstract

We have constructed novel DNA substrates (one inverted and three direct repeats) based on the same 0.6-kb repeat sequence to study deletions and inversions in Saccharomyces cerevisiae. Spontaneous deletions occur six to eight times more frequently than inversions, irrespective of the distance between the repeats. This difference can be explained by the observation that deletion events can be mediated by a recombination mechanism that can initiate within the intervening sequence of the repeats. Spontaneous and double-strand break (DSB)-induced deletions occur as RAD52-dependent and RAD52-independent events. Those deletion events initiated through a DSB in the unique intervening sequence require the Rad1/Rad10 endonuclease only if the break is distantly located from the flanking DNA repeats. We propose that deletions can occur as three types of recombination events: the conservative RAD52-dependent reciprocal exchange and the nonconservative events, one-ended invasion crossover, and single-strand annealing (SSA). We suggest that one-ended invasion is RAD52 dependent, whereas SSA is RAD52 independent. Whereas deletions, like inversions, occur through reciprocal exchange, deletions can also occur through SSA or one-ended invasion. We propose that the contribution of reciprocal exchange and one-ended invasion crossover vs. SSA events to overall spontaneous deletions is a feature specific for each repeat system, determined by the initiation event and the availability of the Rad52 protein. We discuss the role of the Rad1/Rad10 endonuclease on the initial steps of one-ended invasion crossover and SSA as a function of the location of the initiation event relative to the repeats. We also show that the frequency of recombination between repeats is the same independent of their location (whether on circular plasmids, linear minichromosomes, or natural chromosomes) and have similar RAD52 dependence.

Related Genes
RAD1 RAD10 RAD52
MeSH Terms
Chromosome Inversion Crossing Over, Genetic DNA Damage DNA Repair Enzymes DNA-Binding Proteins/genetics Endonucleases/genetics Fungal Proteins/genetics Genes, Fungal/genetics Models, Genetic Mutation Plasmids/genetics Rad52 DNA Repair and Recombination Protein Recombination, Genetic/genetics Repetitive Sequences, Nucleic Acid/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Deletion/genetics Single-Strand Specific DNA and RNA Endonucleases Sister Chromatid Exchange
Chemicals
DNA-Binding Proteins Fungal Proteins RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins Endonucleases RAD1 protein, S cerevisiae RAD10 protein, S cerevisiae Single-Strand Specific DNA and RNA Endonucleases DNA Repair Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prado F
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
Aguilera A
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/139.1.109
Published
1995-01-00
Pages
109-23
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206311
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/139.1.109 PMC Full Text View journal details
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