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PMID: 8913740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of Ty recombination in yeast by cDNA and transcription: role of the RAD1 and RAD52 genes.

Genetics ·Vol. 144 ·No. 3 ·1996-11-00 ·Pages 947-55

Nevo-Caspi Y, Kupiec M

Abstract

In the yeast Saccharomyces cerevisiae ectopic recombination has been shown to occur at high frequencies for artificially created repeats, but at relatively low frequencies for a natural family of repeated sequences, the Ty family. Little is known about the mechanism(s) that prevent recombination between repeated sequences. We have previously shown that nonreciprocal recombination (gene conversion) of a genetically marked Ty can be induced either by the presence of high levels of Ty cDNA or by transcription of the marked Ty from a GAL1 promoter. These two kinds of induction act in a synergistic manner. To further characterize these two kinds of Ty recombination, we have investigated the role played by the RAD52 and RAD1 genes. We have found that the RAD52 and RAD1 gene products are essential to carry out transcription-induced Ty conversion whereas cDNA-mediated conversion can take place in their absence.

MeSH Terms
DNA Repair Enzymes DNA Transposable Elements DNA, Complementary DNA-Binding Proteins/genetics Endonucleases/genetics Fungal Proteins/genetics Gene Expression Regulation, Fungal Rad52 DNA Repair and Recombination Protein Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Complementary DNA-Binding Proteins Fungal Proteins RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins Endonucleases RAD1 protein, S cerevisiae DNA Repair Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nevo-Caspi Y
Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat-Aviv, Israel.
Kupiec M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/144.3.947
Published
1996-11-00
Pages
947-55
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207634
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/144.3.947 PMC Full Text View journal details
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