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PMID: 2676986 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

REV3, a Saccharomyces cerevisiae gene whose function is required for induced mutagenesis, is predicted to encode a nonessential DNA polymerase.

Journal of bacteriology ·Vol. 171 ·No. 10 ·1989-10-00 ·Pages 5659-67

Morrison A, Christensen RB, Alley J, Beck AK, Bernstine EG, Lemontt JF, Lawrence CW

Abstract

We have cloned the REV3 gene of Saccharomyces cerevisiae by complementation of the rev3 defect in UV-induced mutagenesis. The nucleotide sequence of this gene encodes a predicted protein of Mr 172,956 showing significant sequence similarity to Epstein-Barr virus DNA polymerase and to other members of a class of DNA polymerases including human DNA polymerase alpha and yeast DNA polymerase I. REV3 protein shows less sequence identity, and presumably a more distant evolutionary relationship, to the latter two enzymes than they do to each other. Haploids carrying a complete deletion of REV3 are viable. We suggest that induced mutagenesis in S. cerevisiae depends on a specialized DNA polymerase that is not required for other replicative processes. REV3 is located 2.8 centimorgans from CDC60, on chromosome XVI.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular DNA Replication DNA-Directed DNA Polymerase/genetics Genes, Fungal Molecular Sequence Data Mutation Restriction Mapping Saccharomyces cerevisiae/genetics
Chemicals
DNA-Directed DNA Polymerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Morrison A
Department of Biophysics, School of Medicine, University of Rochester, New York 14642.
Christensen R B
Alley J
Beck A K
Bernstine E G
Lemontt J F
Lawrence C W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.10.5659-5667.1989
Published
1989-10-00
Pages
5659-67
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210411
Subset
IM
Grants
NIGMS NIH HHS · GM21858 · United States
NCRR NIH HHS · P41RR01685 · United States
Databases
GENBANK
M29683
External Links
PubMed source DOI: 10.1128/jb.171.10.5659-5667.1989 PMC Full Text View journal details
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