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PMID: 2492497 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 230-7

Larimer FW, Perry JR, Hardigree AA

Abstract

The REV1 gene of Saccharomyces cerevisiae is required for normal induction of mutations by physical and chemical agents. We have determined the sequence of a 3,485-base-pair segment of DNA that complements the rev1-1 mutant. Gene disruption was used to confirm that this DNA contained the REV1 gene. The sequenced segment contains a single long open reading frame, which can encode a polypeptide of 985 amino acid residues. The REV1 transcript is 3.1 kilobase pairs in length. Frameshift mutations introduced into the open reading frame yielded a Rev-phenotype. A base substitution, encoding Gly-193 to Arg-193, was found in this open reading frame in rev1-1. Deletion mutants, lacking segments of the 5' region of REV1, had intermediate mutability relative to REV1 and rev1-1; a complete deletion exhibited lower mutability than rev1-1. REV1 is not an essential gene. An in-frame fusion of the 5' end of the REV1 open reading frame to the lacZ gene produced beta-galactosidase activity constitutively. The predicted REV1 protein is hydrophilic, with a predicted pI of 9.82. No homologies to RAD1, RAD2, RAD3, RAD7, or RAD10 proteins were noted. A 152-residue internal segment displayed 25% identity with UMUC protein.

MeSH Terms
4-Nitroquinoline-1-oxide/pharmacology Amino Acid Sequence Base Sequence Cosmids DNA, Fungal/genetics,isolation & purification Genes, Fungal Hot Temperature Molecular Sequence Data Mutation Restriction Mapping Saccharomyces cerevisiae/drug effects,genetics,radiation effects Transcription, Genetic Ultraviolet Rays beta-Galactosidase/genetics
Chemicals
DNA, Fungal 4-Nitroquinoline-1-oxide beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Larimer F W
Biology Division, Oak Ridge National Laboratory, Tennessee 37831.
Perry J R
Hardigree A A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.230-237.1989
Published
1989-01-00
Pages
230-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209577
Subset
IM
Databases
GENBANK
M22222
External Links
PubMed source DOI: 10.1128/jb.171.1.230-237.1989 PMC Full Text View journal details
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