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PMID: 12509532 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Global identification of target genes regulated by APETALA3 and PISTILLATA floral homeotic gene action.

The Plant cell ·Vol. 15 ·No. 1 ·2003-01-00 ·Pages 207-22

Zik M, Irish VF

Abstract

Identifying the genes regulated by the floral homeotic genes APETALA3 (AP3) and PISTILLATA (PI) is crucial for understanding the molecular mechanisms that lead to petal and stamen formation. We have used microarray analysis to conduct a broad survey of genes whose expression is affected by AP3 and PI activity. DNA microarrays consisting of 9216 Arabidopsis ESTs were screened with probes corresponding to mRNAs from different mutant and transgenic lines that misexpress AP3 and/or PI. The microarray results were further confirmed by RNA gel blot analyses. Our results suggest that AP3 and PI regulate a relatively small number of genes, implying that many genes used in petal and stamen development are not tissue specific and likely have roles in other processes as well. We recovered genes similar to previously identified petal- and stamen-expressed genes as well as genes that were not implicated previously in petal and stamen development. A very low percentage of the genes recovered encoded transcription factors. This finding suggests that AP3 and PI act relatively directly to regulate the genes required for the basic cellular processes responsible for petal and stamen morphogenesis.

MeSH Terms
Arabidopsis/genetics,growth & development Arabidopsis Proteins/genetics,physiology Cell Wall/genetics Flowers/genetics,growth & development Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Homeobox/genetics Genotype MADS Domain Proteins/genetics,physiology Oligonucleotide Array Sequence Analysis/methods Plants, Genetically Modified RNA, Messenger/genetics,metabolism Signal Transduction/genetics,physiology Transcription Factors/genetics
Chemicals
APETALA 3 protein, Arabidopsis Arabidopsis Proteins MADS Domain Proteins PISTILLATA protein, Arabidopsis RNA, Messenger Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zik Moriyah
Department of Molecular, Cellular, and Developmental Biology, Osborn Memorial Laboratories, Yale University, New Haven, Connecticut 06520, USA.
Irish Vivian F
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.006353
Published
2003-01-00
Pages
207-22
Language
English
Region
England
NLM ID
9208688
PMCID
PMC143492
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.006353 PMC Full Text View journal details
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