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PMID: 1255863 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Demonstration of subpopulations of Dane particles.

Journal of virology ·Vol. 17 ·No. 3 ·1976-03-00 ·Pages 885-93

Kaplan PM, Ford EC, Purcell RH, Gerin JL

Abstract

Two populations of Dane particles were isolated from the plasma of individuals carrying hepatitis B surface antigen. These populations had densities in CsCl of 1.22 and 1.20 g/ml. Endogenous DNA polymerase activity was found to be associated only with the heavier of these two populations. Using a positive stain, electron microscopic examination of these particles suggested that the heavier the particle contained nucleic acid in its core whereas the lighter particle appeared empty. Cores isolated from Dane particles with densities of 1.22 and 1.20 g/ml banded in CsCl at densities of 1.36 and 1.30 g/ml, respectively. Endogenous DNA polymerase activity was associated only with the higher density core particles.

MeSH Terms
Centrifugation, Density Gradient DNA Nucleotidyltransferases/metabolism DNA, Viral Hepatitis B Antigens/analysis Plasma/microbiology Viral Proteins/analysis
Chemicals
DNA, Viral Hepatitis B Antigens Viral Proteins DNA Nucleotidyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaplan P M
Ford E C
Purcell R H
Gerin J L
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.17.3.885-893.1976
Published
1976-03-00
Pages
885-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515489
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.17.3.885-893.1976 PMC Full Text View journal details
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