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PMID: 12604590 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A role of topoisomerase II in linking DNA replication to chromosome condensation.

The Journal of cell biology ·Vol. 160 ·No. 5 ·2003-03-03 ·Pages 645-55

Cuvier O, Hirano T

Abstract

The condensin complex and topoisomerase II (topo II) have different biochemical activities in vitro, and both are required for mitotic chromosome condensation. We have used Xenopus egg extracts to investigate the functional interplay between condensin and topo II in chromosome condensation. When unreplicated chromatin is directly converted into chromosomes with single chromatids, the two proteins must function together, although they are independently targeted to chromosomes. In contrast, the requirement for topo II is temporarily separable from that of condensin when chromosome assembly is induced after DNA replication. This experimental setting allows us to find that, in the absence of condensin, topo II becomes enriched in an axial structure within uncondensed chromatin. Subsequent addition of condensin converts this structure into mitotic chromosomes in an ATP hydrolysis-dependent manner. Strikingly, preventing DNA replication by the addition of geminin or aphidicolin disturbs the formation of topo II-containing axes and alters the binding property of topo II with chromatin. Our results suggest that topo II plays an important role in an early stage of chromosome condensation, and that this function of topo II is tightly coupled with prior DNA replication.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Animals Cell Nucleus/enzymology,ultrastructure Chromatin/genetics,metabolism Chromosome Structures/genetics,metabolism DNA Replication/genetics DNA Topoisomerases, Type II/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Eukaryotic Cells/cytology,enzymology Female Male Mitosis/genetics Multiprotein Complexes Nucleic Acid Synthesis Inhibitors/pharmacology Oocytes Spermatozoa Teniposide/pharmacology Xenopus laevis
Chemicals
Chromatin DNA-Binding Proteins Multiprotein Complexes Nucleic Acid Synthesis Inhibitors condensin complexes Teniposide Adenosine Triphosphatases DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cuvier Olivier
Cold Spring Harbor Laboratory, 1 Bungtown Rd., Cold Spring Harbor, NY 11724, USA.
Hirano Tatsuya
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200209023
Published
2003-03-03
Epub
2003-00-25
Pages
645-55
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173373
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200209023 PMC Full Text View journal details
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