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PMID: 12628927 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription factor YY1 functions as a PcG protein in vivo.

The EMBO journal ·Vol. 22 ·No. 6 ·2003-03-17 ·Pages 1347-58

Atchison L, Ghias A, Wilkinson F, Bonini N, Atchison ML

Abstract

Polycomb group (PcG) proteins function as high molecular weight complexes that maintain transcriptional repression patterns during embryogenesis. The vertebrate DNA binding protein and transcriptional repressor, YY1, shows sequence homology with the Drosophila PcG protein, pleiohomeotic (PHO). YY1 might therefore be a vertebrate PcG protein. We used Drosophila embryo and larval/imaginal disc transcriptional repression systems to determine whether YY1 repressed transcription in a manner consistent with PcG function in vivo. YY1 repressed transcription in Drosophila, and this repression was stable on a PcG-responsive promoter, but not on a PcG-non-responsive promoter. PcG mutants ablated YY1 repression, and YY1 could substitute for PHO in repressing transcription in wing imaginal discs. YY1 functionally compensated for loss of PHO in pho mutant flies and partially corrected mutant phenotypes. Taken together, these results indicate that YY1 functions as a PcG protein. Finally, we found that YY1, as well as Polycomb, required the co-repressor protein CtBP for repression in vivo. These results provide a mechanism for recruitment of vertebrate PcG complexes to DNA and demonstrate new functions for YY1.

MeSH Terms
Alcohol Oxidoreductases Animals DNA-Binding Proteins/chemistry,genetics,metabolism Drosophila/embryology Drosophila Proteins/chemistry,genetics,metabolism Embryo, Nonmammalian Erythroid-Specific DNA-Binding Factors Gene Expression Regulation, Developmental Genes, Insect Genes, Reporter Homeodomain Proteins/chemistry,metabolism Larva Models, Biological Mutation Phenotype Phosphoproteins/genetics,metabolism Polycomb Repressive Complex 1 Polycomb-Group Proteins Promoter Regions, Genetic Repressor Proteins/chemistry,metabolism Transcription Factors/metabolism Transgenes YY1 Transcription Factor
Chemicals
DNA-Binding Proteins Drosophila Proteins Erythroid-Specific DNA-Binding Factors Homeodomain Proteins Pc protein, Drosophila Phosphoproteins Polycomb-Group Proteins Repressor Proteins Transcription Factors YY1 Transcription Factor pho protein, Drosophila phol protein, Drosophila Alcohol Oxidoreductases C-terminal binding protein Polycomb Repressive Complex 1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Atchison Lakshmi
Department of Biology, Chestnut Hill College, 9601 Germantown Avenue, Philadelphia, PA 19118, USA. [email protected]
Ghias Ayesha
Wilkinson Frank
Bonini Nancy
Atchison Michael L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg124
Email
[email protected]
Published
2003-03-17
Pages
1347-58
Language
English
Region
England
NLM ID
8208664
PMCID
PMC151054
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042415 · United States
NIDDK NIH HHS · T32 DK007708 · United States
NEI NIH HHS · R29-EY11259 · United States
NIDDK NIH HHS · T32 DK07708 · United States
External Links
PubMed source DOI: 10.1093/emboj/cdg124 PMC Full Text View journal details
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