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PMID: 12631722 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequential entry of components of the gene expression machinery into daughter nuclei.

Molecular biology of the cell ·Vol. 14 ·No. 3 ·2003-03-00 ·Pages 1043-57

Prasanth KV, Sacco-Bubulya PA, Prasanth SG, Spector DL

Abstract

In eukaryotic cells, RNA polymerase II (RNA pol II) transcription and pre-mRNA processing are coordinated events. We have addressed how individual components of the transcription and pre-mRNA processing machinery are organized during mitosis and subsequently recruited into the newly formed daughter nuclei. Interestingly, localization studies of numerous RNA pol II transcription and pre-mRNA processing factors revealed a nonrandom and sequential entry of these factors into daughter nuclei after nuclear envelope/lamina formation. The initiation competent form of RNA pol II and general transcription factors appeared in the daughter nuclei simultaneously, but prior to pre-mRNA processing factors, whereas the elongation competent form of RNA pol II was detected even later. The differential entry of these factors rules out the possibility that they are transported as a unitary complex. Telophase nuclei were competent for transcription and pre-mRNA splicing concomitant with the initial entry of the respective factors. In addition, our results revealed a low turnover rate of transcription and pre-mRNA splicing factors during mitosis. We provide evidence to support a model in which the entry of the RNA pol II gene expression machinery into newly forming daughter nuclei is a staged and ordered process.

MeSH Terms
Cell Nucleus/metabolism Gene Expression Regulation HeLa Cells Humans Mitosis/physiology RNA Polymerase II/metabolism RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA Splicing RNA, Messenger/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
RNA Precursors RNA, Messenger Transcription Factors RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Prasanth Kannanganattu V
Cold Spring Harbor Laboratory, New York 11724, USA.
Sacco-Bubulya Paula A
Prasanth Supriya G
Spector David L
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e02-10-0669
Published
2003-03-00
Pages
1043-57
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC151578
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042694 · United States
PHS HHS · 42694 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1091/mbc.e02-10-0669 PMC Full Text View journal details
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