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PMID: 12692321 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A reevaluation of the role of the heterotrimeric G protein in coupling light responses in Arabidopsis.

Plant physiology ·Vol. 131 ·No. 4 ·2003-04-00 ·Pages 1623-7

Jones AM, Ecker JR, Chen JG

Abstract

Previous studies implicated the involvement of a heterotrimeric G protein in red (R) and far-red (FR) light signal transduction, but these studies utilized pharmacological or gain-of-function approaches and, therefore, are indirect tests. Here, we reexamine the role of the single canonical heterotrimeric G protein in R and FR control of hypocotyl growth using a loss-of-function approach. Single- and double-null mutants for the GPA1, AGB1 genes encoding the alpha and beta subunit of the heterotrimeric G protein, respectively, have wild-type sensitivity to R and FR. Ectopic overexpression of wild type and a constitutive active form of the alpha subunit and of the wild-type beta subunit had no effect that can be unequivocally attributed to altered R and FR responsiveness. These results preclude a direct role for the heterotrimeric G complex in R and FR transduction in Arabidopsis leading to growth control in the hypocotyl.

MeSH Terms
Arabidopsis/genetics,metabolism,radiation effects Arabidopsis Proteins/genetics,metabolism GTP-Binding Protein alpha Subunits GTP-Binding Protein beta Subunits Gene Expression Regulation, Plant Heterotrimeric GTP-Binding Proteins/genetics,metabolism Infrared Rays Light Mutation Phenotype Signal Transduction
Chemicals
AGB1 protein, Arabidopsis Arabidopsis Proteins GPA1 protein, Arabidopsis GTP-Binding Protein alpha Subunits GTP-Binding Protein beta Subunits Heterotrimeric GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jones Alan M
The Salk Institute for Biological Studies, La Jolla, California 92037, USA. [email protected]
Ecker Joseph R
Chen Jin-Gui
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.102.017624
Email
[email protected]
Published
2003-04-00
Pages
1623-7
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC166918
Subset
IM
Grants
NIGMS NIH HHS · R01 GM065989 · United States
NIGMS NIH HHS · GM65989-01 · United States
External Links
PubMed source DOI: 10.1104/pp.102.017624 PMC Full Text View journal details
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