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PMID: 12719597 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication.

Journal of virology ·Vol. 77 ·No. 10 ·2003-05-00 ·Pages 6055-61

Elazar M, Cheong KH, Liu P, Greenberg HB, Rice CM, Glenn JS

Abstract

We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)'s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies.

MeSH Terms
Amino Acid Sequence Cell Line Cell Membrane/metabolism,virology Conserved Sequence Gene Expression Regulation, Viral Hepacivirus/genetics,physiology Humans Peptides/chemical synthesis,chemistry RNA, Viral/biosynthesis Viral Nonstructural Proteins/chemistry,genetics,metabolism Virus Replication
Chemicals
NS-5 protein, hepatitis C virus Peptides RNA, Viral Viral Nonstructural Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Elazar Menashe
Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Palo Alto, California 94305, USA.
Cheong Kwang Ho
Liu Ping
Greenberg Harry B
Rice Charles M
Glenn Jeffrey S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.10.6055-6061.2003
Published
2003-05-00
Pages
6055-61
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC154017
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.77.10.6055-6061.2003 PMC Full Text View journal details
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