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PMID: 12721375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Resistance of cell membranes to different detergents.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 10 ·2003-05-13 ·Pages 5795-800

Schuck S, Honsho M, Ekroos K, Shevchenko A, Simons K

Abstract

Partial resistance of cell membranes to solubilization with mild detergents and the analysis of isolated detergent-resistant membranes (DRMs) have been used operationally to define membrane domains. Given the multitude of detergents used for this purpose, we sought to investigate whether extraction with different detergents might reflect the same underlying principle of domain formation. We therefore compared the protein and lipid content of DRMs prepared with a variety of detergents from two cell lines. We found that the detergents differ considerably in their ability to selectively solubilize membrane proteins and to enrich sphingolipids and cholesterol over glycerophospholipids as well as saturated over unsaturated phosphatidylcholine. In addition, we observed cell type-dependent variations of the molecular characteristics of DRMs and the effectiveness of particular detergents. These results make it unlikely that different detergents reflect the same aspects of membrane organization and underscore both the structural complexity of cell membranes and the need for more sophisticated analytical tools to understand their architecture.

MeSH Terms
Animals Cell Line Cell Membrane/chemistry,drug effects,ultrastructure Cetomacrogol/pharmacology Detergents/pharmacology Dogs Kidney Membrane Lipids/isolation & purification,physiology Membrane Proteins/drug effects,isolation & purification Phospholipids/isolation & purification,physiology Polysorbates/pharmacology Solubility Surface-Active Agents/pharmacology
Chemicals
Detergents Membrane Lipids Membrane Proteins Phospholipids Polysorbates Surface-Active Agents Cetomacrogol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schuck Sebastian
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Honsho Masanori
Ekroos Kim
Shevchenko Andrej
Simons Kai
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0631579100
Published
2003-05-13
Epub
2003-00-29
Pages
5795-800
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC156280
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0631579100 PMC Full Text View journal details
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