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PMID: 12802075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

XBX-1 encodes a dynein light intermediate chain required for retrograde intraflagellar transport and cilia assembly in Caenorhabditis elegans.

Molecular biology of the cell ·Vol. 14 ·No. 5 ·2003-05-00 ·Pages 2057-70

Schafer JC, Haycraft CJ, Thomas JH, Yoder BK, Swoboda P

Abstract

Intraflagellar transport (IFT) is a process required for flagella and cilia assembly that describes the dynein and kinesin mediated movement of particles along axonemes that consists of an A and a B complex, defects in which disrupt retrograde and anterograde transport, respectively. Herein, we describe a novel Caenorhabditis elegans gene, xbx-1, that is required for retrograde IFT and shares homology with a mammalian dynein light intermediate chain (D2LIC). xbx-1 expression in ciliated sensory neurons is regulated by the transcription factor DAF-19, as demonstrated previously for genes encoding IFT complex B proteins. XBX-1 localizes to the base of the cilia and undergoes anterograde and retrograde movement along the axoneme. Disruption of xbx-1 results in cilia defects and causes behavioral abnormalities observed in other cilia mutants. Analysis of cilia in xbx-1 mutants reveals that they are shortened and have a bulb like structure in which IFT proteins accumulate. The role of XBX-1 in IFT was further confirmed by analyzing the effect that other IFT mutations have on XBX-1 localization and movement. In contrast to other IFT proteins, retrograde XBX-1 movement was detected in complex A mutants. Our results suggest that the DLIC protein XBX-1 functions together with the CHE-3 dynein in retrograde IFT, downstream of the complex A proteins.

MeSH Terms
Animals Animals, Genetically Modified Caenorhabditis elegans/genetics,metabolism Cilia/metabolism Dyneins/genetics,metabolism Flagella/metabolism Gene Expression Regulation Genes, Reporter Molecular Motor Proteins/metabolism Sequence Deletion
Chemicals
Molecular Motor Proteins Dyneins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schafer Jenny C
Department of Cell Biology, University of Alabama at Birmingham Medical Center, 35294, USA.
Haycraft Courtney J
Thomas James H
Yoder Bradley K
Swoboda Peter
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e02-10-0677
Published
2003-05-00
Epub
2003-00-26
Pages
2057-70
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC165097
Subset
IM
Grants
NIDDK NIH HHS · R01 DK062758 · United States
NIGMS NIH HHS · R01 GM048700 · United States
NIGMS NIH HHS · R01 GM48700 · United States
NIDDK NIH HHS · R01-DK-62758 · United States
External Links
PubMed source DOI: 10.1091/mbc.e02-10-0677 PMC Full Text View journal details
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