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PMID: 12897126 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 23 ·No. 16 ·2003-08-00 ·Pages 5502-15

Das B, Butler JS, Sherman F

Abstract

A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Delta strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-delta strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-delta, rai1-delta, and cbc1-delta deletions, but not by the upf1-delta deletion, suggesting that DRN requires Rrp6p, a 3'-to-5' nuclear exonuclease, the Rat1p, a 5'-to-3' nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3' end formation defects in certain mutants.

MeSH Terms
Blotting, Northern Cell Nucleus/metabolism Exoribonucleases/metabolism Exosome Multienzyme Ribonuclease Complex Gene Deletion Genotype In Situ Hybridization In Situ Hybridization, Fluorescence Microscopy, Fluorescence Mutation Nuclear Pore Complex Proteins/chemistry Phenotype Poly A Protein Binding RNA, Messenger/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/metabolism Temperature Time Factors Transcription, Genetic
Chemicals
Nuclear Pore Complex Proteins RNA, Messenger Saccharomyces cerevisiae Proteins Poly A Exoribonucleases Exosome Multienzyme Ribonuclease Complex RRP6 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Das Biswadip
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
Butler J Scott
Sherman Fred
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.16.5502-5515.2003
Published
2003-08-00
Pages
5502-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC166345
Subset
IM
Grants
NIGMS NIH HHS · R01 GM012702 · United States
NIGMS NIH HHS · R01 GM059898 · United States
NIGMS NIH HHS · R01 GM12702 · United States
NIGMS NIH HHS · R01 GM59898 · United States
External Links
PubMed source DOI: 10.1128/MCB.23.16.5502-5515.2003 PMC Full Text View journal details
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