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PMID: 1291231 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of DNA synthesis genes in budding yeast: involvement of the transcriptional modulator MOT1 in the expression of the DNA polymerase alpha gene.

Chromosoma ·Vol. 102 ·No. 1 Suppl ·1992-00-00 ·Pages S107-13

Piatti S, Tazzi R, Pizzagalli A, Plevani P, Lucchini G

Abstract

Periodic transcription during the cell cycle of the budding yeast DNA polymerase alpha gene (POL1) requires the cis-acting element 5' ACGCGT 3', which has been found in the 5' non-coding region of all the DNA synthesis genes analyzed so far. Search for trans-acting mutations affecting POL1 expression led to the isolation of the temperature-sensitive reg1033 mutant, that showed increased levels of both DNA polymerase alpha and delta gene transcripts. Cloning of the REG1033 gene demonstrated that it is essential for cell viability and required for proper expression of the POL1 gene. DNA sequence comparison established that the REG1033 gene is identical to MOT1, a gene encoding a presumptive DNA helicase which modulates transcription of several yeast genes.

Related Genes
MOT1 POL1 REG1033
MeSH Terms
Base Sequence Cell Cycle/genetics Cloning, Molecular DNA Polymerase II/genetics DNA Replication/genetics Gene Expression Regulation, Enzymologic/genetics Gene Expression Regulation, Fungal/genetics Molecular Sequence Data Mutation Saccharomyces cerevisiae/genetics Trans-Activators/genetics
Chemicals
Trans-Activators DNA Polymerase II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Piatti S
Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Italy.
Tazzi R
Pizzagalli A
Plevani P
Lucchini G
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26 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF02451793
Published
1992-00-00
Pages
S107-13
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02451793 View journal details
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