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PMID: 1563043 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Positive cis-acting regulatory sequences mediate proper control of POL1 transcription in Saccharomyces cerevisiae.

Current genetics ·Vol. 21 ·No. 3 ·1992-03-00 ·Pages 183-9

Pizzagalli A, Piatti S, Derossi D, Gander I, Plevani P, Lucchini G

Abstract

The 5'ACGCGT3' MluI motif, which is found in the upstream region of several yeast DNA-synthesis genes which are periodically expressed during the mitotic cell-cycle, is present twice in the 5' non-coding region of the DNA-polymerase alpha gene (POL1). Deletion of the most distal repeat does not affect POL1 transcription, while the adjacent 40 base-pair (bp) downstream sequence is necessary both for the proper level and the fluctuation of POL1 mRNA. This region contains the 5'ACGCGTCGCGT3' sequence, which is sufficient to control periodic transcription of a CYC1-lacZ reporter gene with the same kinetics observed for POL1. The adjacent 29 bp AT-rich region does not show any activity by itself, but it acts synergistically in conjunction with at least one MluI hexamer to stimulate CYC1-lacZ expression. By further deletion analysis, DNA sequences necessary to initiate POL1 transcription at the proper sites have also been identified.

Related Genes
CYC1 POL1
MeSH Terms
Base Sequence Cell Cycle/genetics DNA Mutational Analysis DNA Polymerase I/genetics DNA Polymerase II/genetics Molecular Sequence Data Promoter Regions, Genetic/physiology Regulatory Sequences, Nucleic Acid/physiology Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic/physiology
Chemicals
DNA Polymerase I DNA Polymerase II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pizzagalli A
Dipartimento di Genetica e di Biologia dei Microorganismi, Università di Milano, Italy.
Piatti S
Derossi D
Gander I
Plevani P
Lucchini G
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00336839
Published
1992-03-00
Pages
183-9
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
J03268, M83092, S47168, S49998, S50001, S72771, X58277, X64094, X64095, X64096
External Links
PubMed source DOI: 10.1007/BF00336839 View journal details
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