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PMID: 3909103 Published · ppublish English Journal Article

The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage.

Nucleic acids research ·Vol. 13 ·No. 23 ·1985-12-09 ·Pages 8323-37

Barker DG, White JH, Johnston LH

Abstract

The CDC9 gene of Saccharomyces cerevisiae encodes a DNA ligase, and we have determined the nucleotide sequence of a 3.85 kb fragment of DNA which encompasses the convergently transcribed CDC9 and CDC36 genes. S1 nuclease mapping has revealed a major 5' end for the CDC9 mRNA, and one major and one minor site for 3' polyadenylation. These two sites lie within the C-terminal coding region of the CDC36 gene, implying that these two genes are transcribed from overlapping sequences. An interesting structural feature of the CDC9 gene is a series of 6 hexanucleotide repeats (ATGATT) which occur within the 650 bp immediately upstream from the site of transcription initiation. These repeat elements may be implicated in the cell division cycle regulated expression of CDC9. Comparison of the predicted amino acid sequence of the yeast DNA ligase (Mr 84,806) with the sequences of the T4 and T7 bacteriophage DNA ligases reveals little similarity except for a stretch of approximately 45 amino acids, comprising 3 short homologous segments. This region may represent an ATP-binding domain common to polynucleotide ligases.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Chromosome Mapping Codon DNA Ligases/genetics DNA Repair Gene Expression Regulation Genes, Fungal Genetic Linkage Polynucleotide Ligases/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Codon DNA Ligases Polynucleotide Ligases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barker D G
White J H
Johnston L H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.23.8323
Published
1985-12-09
Pages
8323-37
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322137
Subset
IM
Databases
GENBANK
X03246
External Links
PubMed source DOI: 10.1093/nar/13.23.8323 PMC Full Text View journal details
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