Home LiteratureArticle Details
PMID: 12913143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hyperphosphorylation of a mitochondrial protein, prohibitin, is induced by calyculin A in a rice lesion-mimic mutant cdr1.

Plant physiology ·Vol. 132 ·No. 4 ·2003-08-00 ·Pages 1861-9

Takahashi A, Kawasaki T, Wong HL, Suharsono U, Hirano H, Shimamoto K

Abstract

The rice (Oryza sativa) lesion-mimic mutants, cell death and resistance (cdr), show spontaneous cell death on the entire leaf and exhibited significant resistance to the rice blast fungus. Our previous studies showed that CDR1 and CDR2 genes negatively regulated the phosphorylation steps leading to the activation of NADPH oxidase, which is associated with oxidative burst. To identify novel factors involved in the phosphorylation steps, the phosphorylation level of total proteins was compared between cdr mutants and wild type using two-dimensional gel electrophoresis. Here, we show that the phosphorylation level of four proteins in cdr1 was increased as compared with the wild type after calyculin A treatment. Partial amino acid sequences revealed that one of the four proteins is homologous to prohibitin (PHB), which has been shown to be associated with senescence and cell death and to function as a chaperone in the assembly of mitochondrial respiratory chain complex in yeast and mammals. Analysis of green fluorescent protein fusions indicated that rice PHB (OsPHB1) was targeted to mitochondria as found in yeast and mammals, suggesting a possibility that PHB is involved in defense response and/or programmed cell death through the mitochondrial function.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Death Conserved Sequence Genes, Plant/genetics Marine Toxins Mitochondria/metabolism Mitochondrial Proteins/chemistry,metabolism Molecular Sequence Data Mutation/genetics Oryza/cytology,drug effects,genetics,metabolism Oxazoles/pharmacology Phosphorylation/drug effects Prohibitins Proteins/chemistry,metabolism Repressor Proteins Sequence Alignment
Chemicals
Marine Toxins Mitochondrial Proteins Oxazoles Prohibitins Proteins Repressor Proteins calyculin A
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Takahashi Akira
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0101, Japan.
Kawasaki Tsutomu
Wong Hann Ling
Suharsono Utut
Hirano Hisashi
Shimamoto Ko
References (45)
45 references, click to expand
  1. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins.
    Cell. 2000 Jul 7;102(1):43-53 PMID: 10929712
  2. Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death.
    Plant Physiol. 2002 Apr;128(4):1271-81 PMID: 11950976
  3. The mitochondrial PHB complex: roles in mitochondrial respiratory complex assembly, ageing and degenerative disease.
    Cell Mol Life Sci. 2002 Jan;59(1):143-55 PMID: 11852914
  4. Essential role of the small GTPase Rac in disease resistance of rice.
    Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):759-64 PMID: 11149940
  5. Prohibitin and RACK homologues are up-regulated in trypanosomes induced to undergo apoptosis and in naturally occurring terminally differentiated forms.
    Cell Death Differ. 1998 Jul;5(7):615-22 PMID: 10200516
  6. Harpin-induced hypersensitive cell death is associated with altered mitochondrial functions in tobacco cells.
    Mol Plant Microbe Interact. 2000 Feb;13(2):183-90 PMID: 10659708
  7. The prohibitin family of mitochondrial proteins regulate replicative lifespan.
    Curr Biol. 1997 Aug 1;7(8):607-10 PMID: 9259555
  8. Prohibitin family members interact genetically with mitochondrial inheritance components in Saccharomyces cerevisiae.
    Mol Cell Biol. 1998 Jul;18(7):4043-52 PMID: 9632789
  9. Down-regulation of RFL, the FLO/LFY homolog of rice, accompanied with panicle branch initiation.
    Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):1979-82 PMID: 9482818
  10. The oat mitochondrial permeability transition and its implication in victorin binding and induced cell death.
    Plant J. 2002 Feb;29(3):295-312 PMID: 11844107
  11. Membrane protein degradation by AAA proteases in mitochondria: extraction of substrates from either membrane surface.
    Mol Cell. 2000 Apr;5(4):629-38 PMID: 10882099
  12. Proteomic approach to identify novel mitochondrial proteins in Arabidopsis.
    Plant Physiol. 2001 Dec;127(4):1694-710 PMID: 11743114
  13. Prohibitins regulate membrane protein degradation by the m-AAA protease in mitochondria.
    Mol Cell Biol. 1999 May;19(5):3435-42 PMID: 10207067
  14. Mitochondria and apoptosis.
    Science. 1998 Aug 28;281(5381):1309-12 PMID: 9721092
  15. Biochemical pathways of caspase activation during apoptosis.
    Annu Rev Cell Dev Biol. 1999;15:269-90 PMID: 10611963
  16. Animal cell-death suppressors Bcl-x(L) and Ced-9 inhibit cell death in tobacco plants.
    Curr Biol. 1999 Jul 15;9(14):775-8 PMID: 10421577
  17. Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells.
    Mol Cell Biol. 1991 Mar;11(3):1372-81 PMID: 1996099
  18. Cell cycle activity and expression of prohibitin mRNA.
    J Cell Physiol. 1993 Nov;157(2):289-95 PMID: 8227162
  19. Common and contrasting themes of plant and animal diseases.
    Science. 2001 Jun 22;292(5525):2285-9 PMID: 11423652
  20. Plant pathogens and integrated defence responses to infection.
    Nature. 2001 Jun 14;411(6839):826-33 PMID: 11459065
  21. Prohibitin, an antiproliferative protein, is localized to mitochondria.
    FEBS Lett. 1995 Jan 30;358(3):273-7 PMID: 7843414
  22. Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature.
    Plant Physiol. 2001 Apr;125(4):1912-8 PMID: 11299370
  23. Prohibitin and the senescent phenotype.
    Exp Gerontol. 1996 Jan-Apr;31(1-2):245-52 PMID: 8706794
  24. Lesion mimic mutants of rice with alterations in early signaling events of defense.
    Plant J. 1999 Mar;17(5):535-45 PMID: 10205906
  25. Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins.
    EMBO J. 2000 Jun 1;19(11):2444-51 PMID: 10835343
  26. Suramin inhibits initiation of defense signaling by systemin, chitosan, and a beta-glucan elicitor in suspension-cultured Lycopersicon peruvianum cells.
    Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8862-7 PMID: 10922047
  27. Isolation of a cDNA that hybrid selects antiproliferative mRNA from rat liver.
    Biochem Biophys Res Commun. 1989 Nov 15;164(3):1316-22 PMID: 2480116
  28. Apoptotic pathways: paper wraps stone blunts scissors.
    Cell. 2000 Jul 7;102(1):1-4 PMID: 10929706
  29. Perception and response in plant disease resistance.
    Curr Opin Genet Dev. 1994 Oct;4(5):662-71 PMID: 7849505
  30. Reduced levels of chloroplast FtsH protein in tobacco mosaic virus-infected tobacco leaves accelerate the hypersensitive reaction.
    Plant Cell. 2000 Jun;12(6):917-32 PMID: 10852937
  31. The protein phosphatase inhibitor calyculin A mimics elicitor action in plant cells and induces rapid hyperphosphorylation of specific proteins as revealed by pulse labeling with [33P]phosphate.
    Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):952-6 PMID: 11607454
  32. Characterization of the plant homologue of prohibitin, a gene associated with antiproliferative activity in mammalian cells.
    Plant Mol Biol. 1997 Mar;33(4):753-6 PMID: 9132067
  33. Bax-induced cell death in tobacco is similar to the hypersensitive response.
    Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7956-61 PMID: 10393929
  34. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
    Plant J. 1994 Aug;6(2):271-82 PMID: 7920717
  35. Phosphoproteins involved in the signal transduction of cryptogein, an elicitor of defense reactions in tobacco.
    Mol Plant Microbe Interact. 2000 Aug;13(8):821-9 PMID: 10939253
  36. Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation.
    Science. 1984 Nov 30;226(4678):1097-9 PMID: 6093263
  37. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  38. Prohibitins, stomatins, and plant disease response genes compose a protein superfamily that controls cell proliferation, ion channel regulation, and death.
    J Biol Chem. 2000 Sep 22;275(38):29579-86 PMID: 10862763
  39. Programmed cell death, mitochondria and the plant hypersensitive response.
    Nature. 2001 Jun 14;411(6839):848-53 PMID: 11459068
  40. Light-induced phosphorylation of retina-specific polypeptides of Drosophila in vivo.
    Science. 1984 Jan 13;223(4632):184-6 PMID: 6419348
  41. Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBI-1).
    Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12295-300 PMID: 11593047
  42. Analysis of the Arabidopsis mitochondrial proteome.
    Plant Physiol. 2001 Dec;127(4):1711-27 PMID: 11743115
  43. Participation of the mitochondrial permeability transition pore in nitric oxide-induced plant cell death.
    FEBS Lett. 2002 Jan 16;510(3):136-40 PMID: 11801241
  44. Programmed cell death in animal development.
    Cell. 1997 Feb 7;88(3):347-54 PMID: 9039261
  45. Molecular characterization of mitochondrial apoptosis-inducing factor.
    Nature. 1999 Feb 4;397(6718):441-6 PMID: 9989411
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.021733
Published
2003-08-00
Pages
1861-9
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC181272
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.103.021733 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2