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PMID: 12955498 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences.

Molecular genetics and genomics : MGG ·Vol. 270 ·No. 2 ·2003-11-00 ·Pages 173-80

Sarkar A, Sim C, Hong YS, Hogan JR, Fraser MJ, Robertson HM, Collins FH

Abstract

piggyBac is a short inverted-repeat-type DNA transposable element originally isolated from the genome of the moth Trichoplusia ni. It is currently the gene vector of choice for the transformation of various insect species. A few sequences with similarity to piggyBac have previously been identified from organisms such as humans ( Looper), the pufferfish Takifugu rubripes ( Pigibaku), Xenopus ( Tx), Daphnia ( Pokey), and the Oriental fruit fly Bactrocera dorsalis. We have now identified 50 piggyBac-like sequences from publicly available genome sequences and expressed sequence tags (ESTs). This survey allows the first comparative examination of the distinctive piggyBac transposase, suggesting that it might contain a highly divergent DDD domain, comparable to the widespread DDE domain found in many DNA transposases and retroviral integrases which consists of two absolutely conserved aspartic acids separated by about 70 amino acids with a highly conserved glutamic acid about 35 amino acids further away. Many piggyBac-like sequences were found in the genomes of a phylogenetically diverse range of organisms including fungi, plants, insects, crustaceans, urochordates, amphibians, fishes and mammals. Also, several instances of "domestication" of the piggyBac transposase sequence by the host genome for cellular functions were identified. Novel members of the piggyBac family may be useful in genetic engineering of many organisms.

MeSH Terms
Animals Anopheles/genetics DNA Transposable Elements/genetics Evolution, Molecular Genes, Insect Genome, Human Humans Moths/genetics Phylogeny Tetraodontiformes/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sarkar A
Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-0369, USA.
Sim C
Hong Y S
Hogan J R
Fraser M J
Robertson H M
Collins F H
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-003-0909-0
Published
2003-11-00
Epub
2003-00-29
Pages
173-80
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
Grants
NIAID NIH HHS · P01AI45123 · United States
NIAID NIH HHS · U01AI48846 · United States
NIAID NIH HHS · U01AI50687-01 · United States
External Links
PubMed source DOI: 10.1007/s00438-003-0909-0 View journal details
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