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PMID: 12972615 Published · ppublish English Journal Article

Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs.

Molecular and cellular biology ·Vol. 23 ·No. 19 ·2003-10-00 ·Pages 6982-92

Mitchell P, Petfalski E, Houalla R, Podtelejnikov A, Mann M, Tollervey D

Abstract

Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg(2+)-labile factor present in substoichiometric amounts. This protein was identified as the nuclear protein Yhr081p, the homologue of human C1D, which we have designated Rrp47p (for rRNA processing). Immunoprecipitation of epitope-tagged Rrp47p confirmed its interaction with the exosome and revealed its association with Rrp6p, a 3'-->5' exonuclease specific to the nuclear exosome fraction. Northern analyses demonstrated that Rrp47p is required for the exosome-dependent processing of rRNA and small nucleolar RNA (snoRNA) precursors. Rrp47p also participates in the 3' processing of U4 and U5 small nuclear RNAs (snRNAs). The defects in the processing of stable RNAs seen in rrp47-Delta strains closely resemble those of strains lacking Rrp6p. In contrast, Rrp47p is not required for the Rrp6p-dependent degradation of 3'-extended nuclear pre-mRNAs or the cytoplasmic 3'-->5' mRNA decay pathway. We propose that Rrp47p functions as a substrate-specific nuclear cofactor for exosome activity in the processing of stable RNAs.

MeSH Terms
3' Untranslated Regions Alternative Splicing Cell Nucleus/metabolism Exoribonucleases/genetics,isolation & purification,metabolism Humans Mutation RNA/metabolism RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal/metabolism RNA, Ribosomal, 5.8S/metabolism RNA, Small Nuclear/biosynthesis RNA, Small Nucleolar/biosynthesis Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
3' Untranslated Regions RNA Precursors RNA, Ribosomal RNA, Ribosomal, 5.8S RNA, Small Nuclear RNA, Small Nucleolar Saccharomyces cerevisiae Proteins RNA Exoribonucleases Rrp47p protein, human
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mitchell Philip
Wellcome Trust Centre for Cell Biology, Institute for Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom. [email protected]
Petfalski Elisabeth
Houalla Rym
Podtelejnikov Alexandre
Mann Matthias
Tollervey David
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.19.6982-6992.2003
Email
[email protected]
Published
2003-10-00
Pages
6982-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC193929
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.23.19.6982-6992.2003 PMC Full Text View journal details
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